Huachansu injection inhibits metastasis of pancreatic cancer in mice model of human tumor xenograft

BMC Complementary and Alternative Medicine

Research Article

Wei-Dong Shi¹  Lu-Ming Liu¹  Xiao-Yan Zhu¹  Jian-Hua Yin² 

[1] Department of Integrative Oncology, Cancer Hospital, Fudan University, 270 Dong An Road, 200032, Shanghai, China;Department of Oncology, Second TCM Hospital of Guang Dong province, 60 Heng Fu Road, Guang Zhou, Guang Dong province, China;

关键词: Huachansu;    Pancreatic cancer;    Liver metastasis;    VEGF;    MMPs;    CA19-9;   

DOI  :  10.1186/1472-6882-14-483

 received in 2013-11-07, accepted in 2014-09-30,  发布年份 2014

来源: Springer

【 摘 要 】

BackgroundHuachansu injection (HCS) is a water-soluble preparation made from Bufo gargarizans’s skin, which has been widely used in clinics for tumor therapy in China. Though the anti-cancer activity of HCS has been verified through studies in vitro and in vivo, there is little research about its potential anti-metastasis effect. The primary objective of this study was to assess the effects of HCS on both the invasion of pancreatic cancer cells in vitro and on the progression of liver metastasis in vivo in this study.MethodsHCS anti-metastasis potential was accessed using both assay of Cell viability and invasion in vitro, and then further Establishing xenograft model in nude mice. In the cell-based assay, mRNA and protein expression of MMP-2, MMP-9 and VEGF was detected by semi-quantitative RT-PCR and western blotting. In animal experiment, liver metastasis nodules and change of liver-body ratio was observed. Meanwhile, correlation of the CA19-9 and CEA content in serum with the progression of liver metastasis was analyzed.ResultWe observed that HCS prevented the invasion of cancer cells, with inhibiting the expressions of MMP-2 and MMP-9, and reduced not only the number of metastasis nodules but the ratio of liver-body weight as well. Furthermore, HCS decreased the expression of MMP-2, MMP-9 and VEGF in liver metastasis, while also reducing CA19-9 contents in serum. In addition, correlation analysis indicated that the level of CA19-9 in serum was closely related to the number of liver metastasis nodules.ConclusionOur experimental results suggest that HCS has some anti-metastasis potential to suppress the growth of liver metastasis by decreasing the expression of MMP-2 and MMP-9 as well as VEGF.

【 授权许可】

Unknown   
© Yin et al.; licensee BioMed Central. 2014. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.