Molecular analysis of non-O1/non-O139 Vibrio choleraeisolated from hospitalised patients in China

BMC Microbiology

Research Article

Sophie Octavia¹  Ruiting Lan¹  Yun Luo²  Gangqiang Ding²  Zheng Zhang²  Lingling Mei²  Julian Ye²  Dazhi Jin² 

[1] School of Biotechnology and Biomolecular Sciences, University of New South Wales, 2052, Sydney, NSW, Australia;Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou, Zhejiang, China;

关键词: Vibrio cholerae;    Non-O1/non-O139 serogroups;    Pulsed-field gel electrophoresis;    Multilocus sequence typing;    Antibiotic resistance;    Type III secretion system;   

DOI  :  10.1186/1471-2180-13-52

 received in 2012-07-10, accepted in 2013-02-26,  发布年份 2013

来源: Springer

【 摘 要 】

BackgroundCholera is still a significant public health issue in developing countries. The aetiological agent is Vibrio cholerae and only two serogroups, O1 and O139, are known to cause pandemic or epidemic cholera. In contrast, non-O1/non-O139 V. cholerae has only been reported to cause sporadic cholera-like illness and localised outbreaks. The aim of this study was to determine the genetic diversity of non-O1/non-O139 V. cholerae isolates from hospitalised diarrhoeal patients in Zhejiang Province, China.ResultsIn an active surveillance of enteric pathogens in hospitalised diarrhoeal patients, nine non-O1/non-O139 V. cholerae isolates were identified from 746 diarrhoeal stool samples at a rate of 1.2%. These isolates and an additional 31 isolates from sporadic cases and three outbreaks were analysed using pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST). PFGE divided the isolates into 25 PFGE types while MLST divided them into 15 sequence types (STs). A single ST, ST80, was predominant which persisted over several years in different cities and caused two outbreaks in recent years. Antibiotic resistance varied with the majority of the isolates resistant to sulphamethoxazole/trimethoprim and nearly all isolates either resistant or intermediate to erythromycin and rifampicin. None of the isolates carried the cholera toxin genes or toxin co-regulated pilus genes but the majority carried a type III secretion system as the key virulence factor.ConclusionsNon-O1/non-O139 V. cholerae is an important contributor to diarrhoeal infections in China. Resistance to commonly used antibiotics limits treatment options. Continuous surveillance of non-O1/non-O139 V. cholerae is important for control and prevention of diarrhoeal infections.

【 授权许可】

Unknown   
© Luo et al.; licensee BioMed Central Ltd. 2013. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.