Neuroprotective effect of Aronia melanocarpa extract against glutamate-induced oxidative stress in HT22 cells

BMC Complementary and Alternative Medicine

Research Article

Hyeon Yong Lee¹  Myong Ki Kim¹  Gahee Ryu²  Jin Bae Weon²  Nam Young Kim²  Woo Seung Yang²  Choong Je Ma³ 

[1] Department of Food Science and Engineering, Seowon University, 361-742, Cheongju, Republic of Korea;Future Food Research Center, 377-3 Musimsero, Seowon-gu, 28674, Cheongju, Chungbuk, Republic of Korea;Department of Medical Biomaterials Engineering, College of Biomedical Science, Kangwon National University, Hyoja-2 Dong, 200-701, Chuncheon, Republic of Korea;Department of Medical Biomaterials Engineering, College of Biomedical Science, Kangwon National University, Hyoja-2 Dong, 200-701, Chuncheon, Republic of Korea;Institute of Bioscience and Biotechnology, Kangwon National University, 200-701, Chuncheon, Republic of Korea;

关键词: Aronia melanocarpa;    Neuroprotective effect;    Glutamate;    Antioxidant effect;   

DOI  :  10.1186/s12906-017-1716-1

 received in 2016-12-14, accepted in 2017-04-01,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundGlutamate (an endogenous excitatory neurotransmitter) at high concentrations contributes to the development of neurodegenerative diseases. Aronia melanocarpa (A. melanocarpa) berries contain anthocyanins and have high antioxidant activities. In this study, we evaluated whether A. melanocarpa berries could protect neuronal cells against glutamate-induced oxidative stress.MethodA. melanocarpa berries exerted a protective effect against cytotoxicity in HT22 mouse hippocampal cells by MTT assay. We evaluated oxidative stress parameters including ROS level, intracellular Ca2+ level, glutathione level and antioxidant enzyme activity in HT22 cells to elucidate the mechanism of its neuroprotective effect.ResultsA. melanocarpa berries decreased glutamate-induced death of HT22 cells. In addition, A. melanocarpa berries reduced ROS and intracellular Ca2+ levels. Glutathione level, antioxidant enzymes, glutathione reductase and glutathione peroxide activities and mitochondrial membrane potential were also increased in HT22 cells.ConclusionThese results suggested that A. melanocarpa berries protected HT22 cells by exerting an antioxidant effect.

【 授权许可】

CC BY   
© The Author(s). 2017