Dendropanax morbifera Léveille extract facilitates cadmium excretion and prevents oxidative damage in the hippocampus by increasing antioxidant levels in cadmium-exposed rats

BMC Complementary and Alternative Medicine

Research Article

Soon-Min Hong¹  Dong-Woo Kim¹  In Koo Hwang²  Dae Young Yoo²  Yeo Sung Yoon²  Sung Min Nam²  Jong Whi Kim²  Woosuk Kim²  Hyo Young Jung²  Jung Hoon Choi³  Dae Won Kim⁴  Seung Myung Moon⁵ 

[1] Central Research Center, Egreen Co. Ltd, 463-862, Seongnam, South Korea;Department of Anatomy and Cell Biology, and Research Institute for Veterinary Science, College of Veterinary Medicine, Seoul National University, 151-742, Seoul, South Korea;Department of Anatomy, College of Veterinary Medicine, Kangwon National University, 200-701, Chuncheon, South Korea;Department of Biochemistry and Molecular Biology, Research Institute of Oral Sciences, College of Dentistry, Kangneung-Wonju National University, 210-702, Gangneung, South Korea;Department of Neurosurgery, Dongtan Sacred Heart Hospital, College of Medicine, Hallym University, 445-170, Hwaseong, South Korea;

关键词: Dendropanax morbifera;    Cadmium;    Hippocampus;    Oxidative stress;    Antioxidants;   

DOI  :  10.1186/1472-6882-14-428

 received in 2014-10-07, accepted in 2014-10-17,  发布年份 2014

来源: Springer

【 摘 要 】

BackgroundDendropanax morbifera Léveille is used in herbal medicine as a cancer treatment. In this study, we investigated the effects of Dendropanax morbifera stem extract (DMS) on cadmium (Cd) excretion from the blood and kidney and brain tissues of rats exposed to cadmium, as well as the effects of DMS on oxidative stress and antioxidant levels in the hippocampus after Cd exposure.MethodsSeven-week-old Sprague-Dawley rats were exposed to 2 mg/kg of cadmium by intragastric gavage and were orally administered 100 mg/kg of DMS for 4 weeks. Animals were sacrificed and Cd determination was performed using inductively coupled plasma mass spectrometry. In addition, the effects of Cd and/or DMS on oxidative stress were assayed by measuring reactive oxygen species production, protein carbonyl modification, lipid peroxidation levels, and antioxidant levels in hippocampal homogenates.ResultsExposure to Cd significantly increased Cd content in the blood, kidneys, and hippocampi. DMS treatment significantly reduced Cd content in the blood and kidneys, but not in the hippocampi. Exposure to Cd significantly increased reactive oxygen species production, protein carbonyl modification, lipid peroxidation, total sulfhydryl content, reduced glutathione content, and glutathione reductase activity. In contrast, Cu, Zn-superoxide dismutase (SOD1), catalase (CAT), glutathione peroxidase (GPx), and glutathione-S-transferase (GST) activity in the hippocampus were significantly decreased after exposure to Cd, and administration of DMS significantly inhibited these Cd-induced changes.ConclusionThese results indicate that DMS facilitates cadmium excretion from the kidneys, reduces cadmium-induced oxidative stress in the hippocampus, and modulates SOD1, CAT, GPx, and glutathione-S-transferase activities.

【 授权许可】

Unknown   
© Kim et al.; licensee BioMed Central Ltd. 2014. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.