Dendropanax morbifera Léveille extract facilitates cadmium excretion and prevents oxidative damage in the hippocampus by increasing antioxidant levels in cadmium-exposed rats

BMC Complementary and Alternative Medicine

In Koo Hwang⁵  Yeo Sung Yoon⁵  Seung Myung Moon³  Jung Hoon Choi²  Dong-Woo Kim⁴  Soon-Min Hong⁴  Jong Whi Kim⁵  Sung Min Nam⁵  Hyo Young Jung⁵  Dae Young Yoo⁵  Dae Won Kim¹  Woosuk Kim⁵ 

[1] Department of Biochemistry and Molecular Biology, Research Institute of Oral Sciences, College of Dentistry, Kangneung-Wonju National University, Gangneung 210-702, Korea;Department of Anatomy, College of Veterinary Medicine, Kangwon National University, Chuncheon 200-701, South Korea;Department of Neurosurgery, Dongtan Sacred Heart Hospital, College of Medicine, Hallym University, Hwaseong 445-170, South Korea;Central Research Center, Egreen Co. Ltd, Seongnam 463-862, South Korea;Department of Anatomy and Cell Biology, and Research Institute for Veterinary Science, College of Veterinary Medicine, Seoul National University, Seoul 151-742, South Korea

关键词: Antioxidants;    Oxidative stress;    Hippocampus;    Cadmium;    Dendropanax morbifera extract;   

Others  :  1085663
DOI  :  10.1186/1472-6882-14-428

 received in 2014-10-07, accepted in 2014-10-17,  发布年份 2014

【 摘 要 】

Background

Dendropanax morbifera Léveille is used in herbal medicine as a cancer treatment. In this study, we investigated the effects of Dendropanax morbifera stem extract (DMS) on cadmium (Cd) excretion from the blood and kidney and brain tissues of rats exposed to cadmium, as well as the effects of DMS on oxidative stress and antioxidant levels in the hippocampus after Cd exposure.

Methods

Seven-week-old Sprague-Dawley rats were exposed to 2 mg/kg of cadmium by intragastric gavage and were orally administered 100 mg/kg of DMS for 4 weeks. Animals were sacrificed and Cd determination was performed using inductively coupled plasma mass spectrometry. In addition, the effects of Cd and/or DMS on oxidative stress were assayed by measuring reactive oxygen species production, protein carbonyl modification, lipid peroxidation levels, and antioxidant levels in hippocampal homogenates.

Results

Exposure to Cd significantly increased Cd content in the blood, kidneys, and hippocampi. DMS treatment significantly reduced Cd content in the blood and kidneys, but not in the hippocampi. Exposure to Cd significantly increased reactive oxygen species production, protein carbonyl modification, lipid peroxidation, total sulfhydryl content, reduced glutathione content, and glutathione reductase activity. In contrast, Cu, Zn-superoxide dismutase (SOD1), catalase (CAT), glutathione peroxidase (GPx), and glutathione-S-transferase (GST) activity in the hippocampus were significantly decreased after exposure to Cd, and administration of DMS significantly inhibited these Cd-induced changes.

Conclusion

These results indicate that DMS facilitates cadmium excretion from the kidneys, reduces cadmium-induced oxidative stress in the hippocampus, and modulates SOD1, CAT, GPx, and glutathione-S-transferase activities.

【 授权许可】

   
2014 Kim et al.; licensee BioMed Central Ltd.

【 图 表 】

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