Pseudomonas fluorescens SBW25 produces furanomycin, a non-proteinogenic amino acid with selective antimicrobial properties

BMC Microbiology

Research Article

Kerry McPhail¹  Donald Armstrong²  Kristin Trippe³  Mark Azevedo³  Gary Banowetz³ 

[1] College of Pharmacy, Oregon State University, 97331, Corvallis, OR, USA;Department of Botany and Plant Pathology, Oregon State University, 97331, Corvallis, OR, USA;USDA-ARS National Forage Seed Production Research Center, 97331, Corvallis, OR, USA;

关键词: Pseudomonas fluorescens;    Pseudomonas fluorescens;    Secondary metabolites;    Non-proteinogenic amino acids;    Antimicrobial activity;    4-formylaminooxyvinylglycine;    Furanomycin;   

DOI  :  10.1186/1471-2180-13-111

 received in 2012-12-24, accepted in 2013-05-07,  发布年份 2013

来源: Springer

【 摘 要 】

BackgroundPseudomonas fluorescens SBW25 has been extensively studied because of its plant growth promoting properties and potential as a biocontrol agent. The genome of SBW25 has been sequenced, and among sequenced strains of pseudomonads, SBW25 appears to be most closely related to P. fluorescens WH6. In the authors’ laboratories, WH6 was previously shown to produce and secrete 4-formylaminooxyvinylglycine (FVG), a non-proteinogenic amino acid with selective herbicidal and antimicrobial activity. Although SBW25 does not have the genetic capacity to produce FVG, we were interested in determining whether this pseudomonad might produce some other type of non-proteinogenic amino acid.ResultsP. fluorescens SBW25 was found to produce and secrete a ninhydrin-reactive compound with selective antimicrobial properties. This compound was purified from SBW25 culture filtrate and identified as the non-proteinogenic amino acid L-furanomycin [2S,2′R,5′S)-2-amino-2-(5′methyl-2′,5′-dihydrofuran-2′-yl)acetic acid].ConclusionsThe identification of furanomycin as a secondary metabolite of SBW25 is the first report of the production of furanomycin by a pseudomonad. This compound was known previously only as a natural product produced by a strain of Streptomyces. This report adds furanomycin to the small list of non-proteinogenic amino acids that have been identified as secondary products of pseudomonads. This study also extends the list of bacteria that are inhibited by furanomycin to include several plant pathogenic bacteria.

【 授权许可】

Unknown   
© Trippe et al.; licensee BioMed Central Ltd. 2013. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.