Chemical composition and biological activity of Rubus idaeus shoots – a traditional herbal remedy of Eastern Europe

BMC Complementary and Alternative Medicine

Research Article

Anna Kawiak¹  Weronika Kozłowska²  Adam Matkowski²  Rafał Hałasa³  Mirosława Krauze-Baranowska⁴  Daniel Głód⁴  Marta Kula⁴  Magdalena Majdan⁴ 

[1] Department of Biotechnology, Division of Plant Protection and Biotechnology, Intercollegiate Faculty of Biotechnology, University of Gdańsk and Medical University of Gdańsk, Kładki Str. 24, 80-822, Gdańsk, Poland;Department of Pharmaceutical Biology and Botany, Wrocław Medical University, Borowska Str. 211, 50-556, Wrocław, Poland;Department of Pharmaceutical Microbiology, Faculty of Pharmacy with Subfaculty of Laboratory Medicine, Medical University of Gdańsk, Gen. J. Hallera Str. 107, 80-416, Gdańsk, Poland;Department of Pharmacognosy with Medicinal Plants Garden, Faculty of Pharmacy with Subfaculty of Laboratory Medicine, Medical University of Gdańsk, Gen. J. Hallera Str. 107, 80-416, Gdańsk, Poland;

关键词: Rubus idaeus;    Shoots;    HPLC-DAD-ESI-MS;    Comprehensive LCxLC;    Biological activity;   

DOI  :  10.1186/1472-6882-14-480

 received in 2014-07-29, accepted in 2014-12-09,  发布年份 2014

来源: Springer

【 摘 要 】

BackgroundThe young shoots of Rubus idaeus are traditionally used as a herbal remedy in common cold, fever and flu-like infections yet there is no research concerning this plant material. The aim of the study was to evaluate the chemical composition and biological properties of raspberry shoots from 11 cultivar varieties.MethodsThe methanol extracts were subjected to chromatographic analysis using HPLC-DAD-ESI-MS, and two-dimensional ‘comprehensive’ LCxLC techniques. The biological activity of the shoot extract from the ‘Willamette’ cultivar variety was evaluated. Antioxidant activity was tested using DPPH and phosphomolybdenum assay. Antimicrobial activity was estimated towards 15 strains of human pathogenic bacteria using broth microdilution method. Cytotoxic activity was tested using MTT cell viability assay.ResultsThe dominating compounds identified in the shoots of R. idaeus were ellagic acid (26.1 - 106.8 mg/100 g) and sanguiin H-6 (139.2 - 633.1 mg/100 g). The best separation of compounds present in the analysed polyphenol complex, was achieved by ‘comprehensive’ LCxLC method using Nucleodur Sphinx RP column in the first dimension and Chromolith Performance column in the second dimension. The shoot extract was found to be a strong antioxidant (EC50 19.4 μg/ml, AAE 427.94 mg/g) and displayed the strongest bactericidal properties towards Corynebacterium diphtheriae. The extract revealed higher cytotoxic activity towards the HL-60 cells (IC50 110 μg/ml) than HeLa (IC50 300 μg/ml).ConclusionsThe shoots of R. idaeus stand out as a valuable source of sanguiin H-6 and ellagic acid and possess a number of biological properties including antioxidative, antimicrobial and cytotoxic.

【 授权许可】

CC BY   
© Krauze-Baranowska et al.; licensee BioMed Central. 2014