Chemical composition and biological activity of Rubus idaeus shoots – a traditional herbal remedy of Eastern Europe

BMC Complementary and Alternative Medicine

Anna Kawiak⁴  Weronika Kozłowska¹  Adam Matkowski¹  Rafał Hałasa³  Magdalena Majdan²  Marta Kula²  Daniel Głód²  Mirosława Krauze-Baranowska² 

[1] Department of Pharmaceutical Biology and Botany, Wrocław Medical University, Borowska Str. 211, 50-556 Wrocław, Poland;Department of Pharmacognosy with Medicinal Plants Garden, Faculty of Pharmacy with Subfaculty of Laboratory Medicine, Medical University of Gdańsk, Gen. J. Hallera Str. 107, 80-416 Gdańsk, Poland;Department of Pharmaceutical Microbiology, Faculty of Pharmacy with Subfaculty of Laboratory Medicine, Medical University of Gdańsk, Gen. J. Hallera Str. 107, 80-416 Gdańsk, Poland;Department of Biotechnology, Division of Plant Protection and Biotechnology, Intercollegiate Faculty of Biotechnology, University of Gdańsk and Medical University of Gdańsk, Kładki Str. 24, 80-822 Gdańsk, Poland

关键词: Biological activity;    Comprehensive LCxLC;    HPLC-DAD-ESI-MS;    Shoots;    Rubus idaeus;   

Others  :  1085023
DOI  :  10.1186/1472-6882-14-480

 received in 2014-07-29, accepted in 2014-12-09,  发布年份 2014

【 摘 要 】

Background

The young shoots of Rubus idaeus are traditionally used as a herbal remedy in common cold, fever and flu-like infections yet there is no research concerning this plant material. The aim of the study was to evaluate the chemical composition and biological properties of raspberry shoots from 11 cultivar varieties.

Methods

The methanol extracts were subjected to chromatographic analysis using HPLC-DAD-ESI-MS, and two-dimensional ‘comprehensive’ LCxLC techniques. The biological activity of the shoot extract from the ‘Willamette’ cultivar variety was evaluated. Antioxidant activity was tested using DPPH and phosphomolybdenum assay. Antimicrobial activity was estimated towards 15 strains of human pathogenic bacteria using broth microdilution method. Cytotoxic activity was tested using MTT cell viability assay.

Results

The dominating compounds identified in the shoots of R. idaeus were ellagic acid (26.1 - 106.8 mg/100 g) and sanguiin H-6 (139.2 - 633.1 mg/100 g). The best separation of compounds present in the analysed polyphenol complex, was achieved by ‘comprehensive’ LCxLC method using Nucleodur Sphinx RP column in the first dimension and Chromolith Performance column in the second dimension. The shoot extract was found to be a strong antioxidant (EC₅₀ 19.4 μg/ml, AAE 427.94 mg/g) and displayed the strongest bactericidal properties towards Corynebacterium diphtheriae. The extract revealed higher cytotoxic activity towards the HL-60 cells (IC₅₀ 110 μg/ml) than HeLa (IC₅₀ 300 μg/ml).

Conclusions

The shoots of R. idaeus stand out as a valuable source of sanguiin H-6 and ellagic acid and possess a number of biological properties including antioxidative, antimicrobial and cytotoxic.

【 授权许可】

   
2014 Krauze-Baranowska et al.; licensee BioMed Central.

【 图 表 】

Figure 1.

Figure 2.

Figure 3.

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