Gene-centromere mapping in meiotic gynogenetic European seabass

BMC Genomics

Research Article

Julie Colléter¹  Stefano Peruzzi²  Béatrice Chatain³  Marc Vandeputte⁴  Münevver Oral⁵  John B Taggart⁵  Christos Palaiokostas⁵  Michaël Bekaert⁵  Brendan J. McAndrew⁵  David J Penman⁵  Heiner Kuhl⁶  Richard Reinhardt⁷ 

[1] Cirad, Persyst, UMR Intrepid, Campus International de Baillarguet, 34398, Montpellier, France;Ifremer, 34250, Palavas-Les-Flots, France;Department of Arctic and Marine Biology, Faculty of Biosciences, Fisheries and Economics, University of Tromsø, 9037, Tromsø, Breivika, Norway;Ifremer, 34250, Palavas-Les-Flots, France;Ifremer, 34250, Palavas-Les-Flots, France;INRA, GABI, AgroParisTech, Université Paris-Saclay, 78350, Jouy-en-Josas, France;Institute of Aquaculture, School of Natural Sciences, University of Stirling, FK9 4LA, Stirling, Scotland, UK;Leibniz-Institute of Freshwater Biology and Inland Fisheries, Müggelseedamm 310, 12587, Berlin, Germany;Max-Planck-Institute for Plant Breeding, Max-Planck Genome Centre Cologne, Carl-von-Linné-Weg 10, D-50829, Cologne, Germany;

关键词: Dicentrarchus labrax;    Meiotic gynogenesis;    Isogenic lines;    ddRAD seq;    Genetic map;    Gene-Centromere map;    Aquaculture;   

DOI  :  10.1186/s12864-017-3826-z

 received in 2016-12-22, accepted in 2017-05-28,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundFully isogenic lines in fish can be developed using “mitotic” gynogenesis (suppression of first zygotic mitosis following inactivation of the sperm genome). However, genome-wide verification of the steps in this process has seldom been applied. We used ddRADseq to generate SNP markers in a meiotic gynogenetic family of European seabass (Dicentrarchus labrax): (i) to verify the lack of paternal contribution in a meiotic gynogenetic family; (ii) to generate a gene-centromere map from this family; (iii) to identify telomeric markers that could distinguish mitotic gynogenetics from meiotic gynogenetics, which sometimes arise spontaneously in mitotic gynogenetic families.ResultsFrom a single meiotic gynogenetic family consisting of 79 progeny, 42 million sequencing reads (Illumina, trimmed to 148 bases) resolved 6866 unique RAD-tags. The 340 male-informative SNP markers that were identified confirmed the lack of paternal contribution. A gene-centromere map was constructed based on 804 female-informative SNPs in 24 linkage groups (2n = 48) with a total length of 1251.02 cM (initial LG assignment was based on the seabass genome assembly, dicLab v1). Chromosome arm structure could be clearly discerned from the pattern of heterozygosity in each linkage group in 18 out of 24 LGs: the other six showed anomalies that appeared to be related to issues in the genome assembly.ConclusionGenome-wide screening enabled substantive verification of the production of the gynogenetic family used in this study. The large number of telomeric and subtelomeric markers with high heterozygosity values in the meiotic gynogenetic family indicate that such markers could be used to clearly distinguish between meiotic and mitotic gynogenetics.

【 授权许可】

CC BY   
© The Author(s). 2017