Dissemination of VIM-2 producing Pseudomonas aeruginosa ST233 at tertiary care hospitals in Egypt

BMC Infectious Diseases

Research Article

Hadir Ahmed El-Mahallawy¹  Mai Mahmoud Zafer²  Seif El Din Ashour³  Magdy Aly Amin⁴  Mohamed Hamed Al-Agamy⁵ 

[1] Department of Clinical Pathology, National Cancer Institute, Cairo University, Cairo, Egypt;Department of Microbiology and Immunology, Faculty of Pharmacy, Ahram Canadian University, 4th Industrial Zone, Banks Complex، 6th of October, Giza, Egypt;Department of Microbiology and Immunology, Faculty of Pharmacy, Al-Azhar University, Cairo, Egypt;Department of Microbiology and Immunology, Faculty of pharmacy, Cairo University, El Aini, As Sayedah Zeinab, Cairo, Egypt;Department of Pharmaceutics and Microbiology, College of Pharmacy, King Saud University, 11451, Riyadh, Saudi Arabia;Department of Microbiology and Immunology, Faculty of Pharmacy, Al-Azhar University, Cairo, Egypt;

关键词: Pseudomonas aeruginosa;    Carbapenem resistance;    bla;    ST233;    Egypt;   

DOI  :  10.1186/s12879-015-0861-8

 received in 2014-09-27, accepted in 2015-03-02,  发布年份 2015

来源: Springer

【 摘 要 】

BackgroundPseudomonas aeruginosa is an important nosocomial pathogen, commonly causing infections in immunocompromised patients. The aim of this study was to examine the genetic relatedness of metallo-beta-lactamase (MBL) producing carbapenem resistant Pseudomonas aeruginosa clinical isolates collected from 2 tertiary hospitals in Cairo, Egypt using Multi Locus sequence typing (MLST).MethodsPhenotypic and genotypic detection of metallo-beta-lactamase for forty eight non-duplicate carbapenem resistant P. aeruginosa isolates were carried out. DNA sequencing and MLST were done.ResultsThe blaVIM-2 gene was highly prevalent (28/33 strains, 85%) among 33 MBL-positive P.aeruginosa isolates. MLST revealed eleven distinct Sequence Types (STs). A unique ST233 clone producing VIM-2 was documented by MLST in P.aeruginosa strains isolated from Cairo university hospitals. The high prevalence of VIM-2 producers was not due to the spread of a single clone.ConclusionsThe findings of the present study clearly demonstrate that clones of VIM-2 positive in our hospitals are different from those reported from European studies. Prevalence of VIM-2 producers of the same clone was detected from surgical specimens whereas oncology related specimens were showing diverse clones.

【 授权许可】

CC BY   
© Zafer et al.; licensee BioMed Central. 2015