Genomic characterization of a large plasmid containing a blaNDM-1 gene carried on Salmonella enterica serovar Indiana C629 isolate from China

BMC Infectious Diseases

Research Article

Zulqarnain Baloch¹  Jin Xu²  Wei Wang²  Zixin Peng²  Yujie Hu²  Fengqin Li³  Séamus Fanning⁴ 

[1] College of Veterinary Medicine, South China Agricultural University, 510642, Guangzhou, People’s Republic of China;Key Laboratory of Food Safety Risk Assessment, Ministry of Health, China National Center for food safety Risk Assessment, Beijing, People’s Republic of China;Key Laboratory of Food Safety Risk Assessment, Ministry of Health, China National Center for food safety Risk Assessment, Beijing, People’s Republic of China;Microbiology Laboratory, China National Centre for Food Safety Risk Assessment, No.7 Panjiayuan Nanli, Chaoyang District, 100021, Beijing, People’s Republic of China;Key Laboratory of Food Safety Risk Assessment, Ministry of Health, China National Center for food safety Risk Assessment, Beijing, People’s Republic of China;UCD-Centre for Food Safety, School of Public Health, Physiotherapy and Sports Science, University College Dublin, Belfield D04 N2E5, Dublin, Ireland;Institute for Global Food Security, School of Biological Sciences, Queen’s University Belfast, Stranmillis Road, BT9 5AG, Belfast, Northern Ireland;

关键词: Extensively-drug resistance;    Salmonella enterica;    Carbapenem resistance;    bla;    Chicken carcass;    China;   

DOI  :  10.1186/s12879-017-2515-5

 received in 2017-03-19, accepted in 2017-06-01,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundThe blaNDM-1 gene in Salmonella species is mostly reported in clinical cases, but is rarely isolated from red and white meat in China.MethodsA Salmonella Indiana (S. Indiana) isolate was cultured from a chicken carcass procured from a slaughterhouse in China. Antimicrobial susceptibility was tested against a panel of agents. Whole-genome sequencing of the isolate was carried out and data was analyzed.ResultsA large plasmid, denoted as plasmid pC629 (210,106 bp), containing a composite cassette, consisting of IS26-blaNDM-1-bleMBL-△trpF-tat-cutA-ISCR1-sul1-qacE△1-aadA2-dfrA12-intI1-IS26 was identified. The latter locus was physically linked with blaOXA-1, blaCTX-M-65, blaTEM-1-encoding genes. A mercury resistance operon merACDEPTR was also identified; it was flanked on the proximal side, among IS26 element and the distally located on the blaNDM-1 gene. Plasmid pC629 also contained 21 other antimicrobial resistance-encoding genes, such as aac(6′)-Ib-cr, aac(3)-VI, aadA5, aph(4)-Ia, arr-3, blmS, brp, catB3, dfrA17, floR, fosA, mph(A), mphR, mrx, nimC/nimA, oqxA, oqxB, oqxR, rmtB, sul1, sul2. Two virulence genes were also identified on plasmid pC629.ConclusionTo the best of our knowledge, this is the first report of blaNDM-1 gene being identified from a plasmid in a S. Indiana isolate cultured from chicken carcass in China.

【 授权许可】

CC BY   
© The Author(s). 2017