Oridonin alters the expression profiles of MicroRNAs in BxPC-3 human pancreatic cancer cells

BMC Complementary and Alternative Medicine

Research Article

Bin Xu¹  Jian Xu²  Shuquan Li²  Zhifang Gui²  Xing Liu³ 

[1] Department of General Surgery, Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, 310016, Hangzhou, China;Medical Technology College, Zhejiang Chinese Medical University, Binwen Road, Binjiang District, 310053, Hangzhou, Zhejiang Province, China;School of Medicine, Jinggangshan University, 343000, Ji’an, China;

关键词: Oridonin;    miRNA;    microarray;    BxPC-3 pancreatic cancer cell;   

DOI  :  10.1186/s12906-015-0640-5

 received in 2014-09-30, accepted in 2015-03-30,  发布年份 2015

来源: Springer

【 摘 要 】

BackgroundOridonin, an ingredient used in traditional Chinese medicine, has been demonstrated to play an important role in antitumour effects, but the mechanism underlying its antitumour properties is still not clear.MethodsTo verify the anti-cancer effects of oridonin via a miRNA-dependent mechanism, comprehensive miRNA expression profiling of oridonin-treated BxPC-3 human pancreatic cancer cells was performed using a miRNA microarray assay based on Sanger miR-Base Release 20, followed by a validation using real-time PCR. MicroRNA target prediction and Gene Ontology and KEGG pathway analysis were performed to investigate possible pathways involved.ResultsThe results showed that 105 miRNAs were significantly differentially expressed (signal reading >500, p ≤ 0.01, |Log2-value| ≥1) in oridonin-treated BxPC-3 human pancreatic cancer cells.ConclusionsOur data indicates that oridonin inhibits BxPC-3 cells probably through regulating the expression of miRNAs. Interruption of miRNA profiling may provide new therapeutic methods for the clinical treatment of pancreatic cancer.

【 授权许可】

Unknown   
© Gui et al.; licensee BioMed Central. 2015. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.