Contrasting nitrogen fertilization treatments impact xylem gene expression and secondary cell wall lignification in Eucalyptus

BMC Plant Biology

Research Article

Paulo José Pereira Lima Teixeira¹  Gonçalo Amarante Guimarães Pereira¹  Jorge Lepikson-Neto¹  Wesley Leoricy Marques¹  Marcelo Falsarella Carazzolle¹  Leandro Costa Nascimento¹  Marcela Mendes Salazar¹  Ana Carolina Deckmann¹  Eduardo Leal Oliveira Camargo²  Yves Martinez³  Marçal Soler⁴  Jacqueline Grima-Pettenati⁴  Ana Alves⁵  José Carlos Rodrigues⁵  Piotr Mieczkowski⁶ 

[1] Departamento de Genética, Evolução e Bioagentes; Laboratório de Genômica e Expressão, Universidade Estadual de Campinas; UNICAMP, Instituto de Biologia, Campinas, Brazil;Departamento de Genética, Evolução e Bioagentes; Laboratório de Genômica e Expressão, Universidade Estadual de Campinas; UNICAMP, Instituto de Biologia, Campinas, Brazil;Laboratoire de Recherche en Sciences Végétales, UMR 5546: CNRS - Université de Toulouse III (UPS), BP 42617, F-31326, Auzeville, Castanet-Tolosan, France;Fédération de Recherche “Agrobiosciences, Interactions et Biodiversité”, BP 42617, 24 Chemin de borde rouge, 31326, Castanet-Tolosan, France;Laboratoire de Recherche en Sciences Végétales, UMR 5546: CNRS - Université de Toulouse III (UPS), BP 42617, F-31326, Auzeville, Castanet-Tolosan, France;Tropical Research Institute of Portugal (IICT), Forestry and Forest Products Group, Tapada da Ajuda, Lisboa, Portugal;Centro de Estudos Florestais, Tapada da Ajuda, Lisboa, Portugal;University of North Carolina at Chapel Hill, Chapel Hill, NC, USA;

关键词: Eucalyptus;    Lignin;    Lignocellulosic biomass;    Nitrogen fertilization;    Wood;    RNA sequencing;   

DOI  :  10.1186/s12870-014-0256-9

 received in 2014-06-11, accepted in 2014-09-20,  发布年份 2014

来源: Springer

【 摘 要 】

BackgroundNitrogen (N) is a main nutrient required for tree growth and biomass accumulation. In this study, we analyzed the effects of contrasting nitrogen fertilization treatments on the phenotypes of fast growing Eucalyptus hybrids (E. urophylla x E. grandis) with a special focus on xylem secondary cell walls and global gene expression patterns.ResultsHistological observations of the xylem secondary cell walls further confirmed by chemical analyses showed that lignin was reduced by luxuriant fertilization, whereas a consistent lignin deposition was observed in trees grown in N-limiting conditions. Also, the syringyl/guaiacyl (S/G) ratio was significantly lower in luxuriant nitrogen samples. Deep sequencing RNAseq analyses allowed us to identify a high number of differentially expressed genes (1,469) between contrasting N treatments. This number is dramatically higher than those obtained in similar studies performed in poplar but using microarrays. Remarkably, all the genes involved the general phenylpropanoid metabolism and lignin pathway were found to be down-regulated in response to high N availability. These findings further confirmed by RT-qPCR are in agreement with the reduced amount of lignin in xylem secondary cell walls of these plants.ConclusionsThis work enabled us to identify, at the whole genome level, xylem genes differentially regulated by N availability, some of which are involved in the environmental control of xylogenesis. It further illustrates that N fertilization can be used to alter the quantity and quality of lignocellulosic biomass in Eucalyptus, offering exciting prospects for the pulp and paper industry and for the use of short coppices plantations to produce second generation biofuels.

【 授权许可】

Unknown   
© Camargo et al.; licensee BioMed Central Ltd. 2014. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.