Ethyl acetate extract of Wedelia chinensis inhibits tert-butyl hydroperoxide-induced damage in PC12 cells and D-galactose-induced neuronal cell loss in mice

BMC Complementary and Alternative Medicine

Research Article

Yean-Jang Lee¹  Hsing-Chun Kuo²  Wea-Lung Lin³  Shao-Ming Wang⁴  Tsui-Hwa Tseng⁵  Ying-Jui Ho⁶ 

[1] Department of Chemistry, National Changhua University of Education, No. 1, Jin-De Road, 500, Changhua, Taiwan;Department of Nursing, Chang Gung University of Science and Technology, Chia-Yi Campus No. 2, Chia-Pu Rd. West Sec. Putz, 613, Chia-Yi, Taiwan;Department of Pathology, Chung Shan Medical University Hospital, No. 110, Section 1, Chien-Kuo N. Road, 402, Taichung, Taiwan;School of Medicine, Chung Shan Medical University, No. 110, Section 1, Chien-Kuo N. Road, Taichung, Taiwan;School of Medical Applied Chemistry, Chung Shan Medical University, No. 110, Section 1, Chien-Kuo N. Road, 402, Taichung, Taiwan;School of Medical Applied Chemistry, Chung Shan Medical University, No. 110, Section 1, Chien-Kuo N. Road, 402, Taichung, Taiwan;Department of Medical Education, Chung Shan Medical University Hospital, No. 110, Section 1, Chien-Kuo N. Road, 402, Taichung, Taiwan;School of Psychology, Chung Shan Medical University, No. 110, Section 1, Chien-Kuo N. Road, 402, Taichung, Taiwan;

关键词: Apoptosis;    t-butylhydroperoxide;    D-galactose;    Luteolin;    Wedelia chinensis;    Wedelolactone;   

DOI  :  10.1186/1472-6882-14-491

 received in 2014-06-13, accepted in 2014-12-10,  发布年份 2014

来源: Springer

【 摘 要 】

BackgroundWedelia chinensis is traditionally used as a hepatoprotective herb in Taiwan. The aim of this study was to evaluate the neuroprotective potential of W. chinensis.MethodsAn ethyl acetate extract of W. chinensis (EAW) was prepared and analyzed by HPLC. The neuroprotective potential of EAW was assessed by tert-butylhydroperoxide (t-BHP)-induced damage in PC12 cells and D-galactose-induced damage in mouse cortex.ResultsEAW exhibited potent radical scavenging property and highly contained luteolin and wedelolactone. EAW decreased t-BHP-induced reactive oxygen species (ROS) accumulation, cytotoxicity and apoptosis in PC12 cells. EAW and its major constituents blocked t-BHP-induced cytochrome C release and Bcl-2 family protein ratio change. EAW and its major constituents increased the endogenous antioxidant capacity evaluated by the binding activity assay of nuclear factor E2-related factor 2 (Nrf2) to antioxidant response element (ARE) and nuclear translocation of Nrf2 respectively in PC12 cells. Finally, EAW inhibited D-galactose-induced lipid peroxidation, apoptosis and neuron loss in the cerebral cortex of mice.ConclusionThese results demonstrate that W. chinensis has neuroprotective potential through blocking oxidative stress-induced damage and that luteolin and wedelolactone contribute to the protective action.

【 授权许可】

Unknown   
© Lin et al.; licensee BioMed Central. 2014. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.