Protective effects of molecular hydrogen on steroid-induced osteonecrosis in rabbits via reducing oxidative stress and apoptosis

BMC Musculoskeletal Disorders

Research Article

Zhaogang Ge¹  Jia Li²  Lihong Fan³  Kunzheng Wang³ 

[1] Department of Joint Surgery, Honghui Hospital of Xi’an Jiaotong University, 710054, Xi’an, Shaanxi Province, People’s Republic of China;Department of Orthopedics, The First Affiliated Hospital of Xi’an Jiaotong University, Yanta West Road, 710061, Xi’an, Shaanxi Province, People’s Republic of China;The first department of Orthopedics, The Second Affiliated Hospital of Xi’an Jiaotong University, 710004, Xi’an, Shaanxi Province, People’s Republic of China;

关键词: Molecular hydrogen;    Osteonecrosis;    Oxidative injury;    Vascular injury;    Apoptosis;   

DOI  :  10.1186/s12891-017-1431-6

 received in 2016-06-09, accepted in 2017-01-24,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundThe objective of this study was to investigate the protective effects of molecular hydrogen, a novel and selective antioxidant, on steroid-induced osteonecrosis (ON) in a rabbit model.MethodsSixty rabbits were randomly divided into two groups (model group and hydrogen group). Osteonecrosis was induced according to an established protocol of steroid-induced ON. Rabbits in the hydrogen group were treated with intraperitoneal injections of molecular hydrogen at 10 ml/kg body weight for seven consecutive days. Plasma levels of total cholesterol, triglycerides, soluble thrombomodulin(sTM), glutathione(GSH) and malondialdehyde(MDA) were measured before and after steroid administration. The presence or absence of ON was examined histopathologically. Oxidative injury and vascular injury were assessed in vivo by immunohistochemical staining of 8-hydoxy-2-deoxyguanosine(8-OHdG) and MDA, and ink artery infusion angiography. The terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) assays were performed to measure apoptosis.ResultsThe incidence of steroid-induced ON was significantly lower in hydrogen group (28.6%) than that in model group (68.0%). No statistically differences were observed on the levels of total cholesterol and triglycerides. Oxidative injury, vascular injury and apoptosis were attenuated in the hydrogen group compared with those in the model group in vivo.ConclusionsThese results suggested that molecular hydrogen prevents steroid-induced osteonecrosis in rabbits by suppressing oxidative injury, vascular injury and apoptosis.

【 授权许可】

CC BY   
© The Author(s). 2017