A Chinese herbal formula "Gan-Lu-Yin" suppresses vascular smooth muscle cell migration by inhibiting matrix metalloproteinase-2/9 through the PI3K/AKT and ERK signaling pathways

BMC Complementary and Alternative Medicine

Research Article

Hsu-Chen Cheng¹  Yi-Chung Chien¹  Po-Liang Cheng¹  Ming-Jyh Sheu²  Wen-Hsin Lin²  Ying-Yi Chen²  Chieh-Hsi Wu² 

[1] Department of Life Sciences, and Agricultural Biotechnology Center, National Chung Hsing University, 250 Kuo Kuang Road, 402, Taichung, Taiwan;School of Pharmacy, China Medical University, 91 Hsueh-Shih Road, 404, Taichung, Taiwan;

关键词: Chinese herbs;    Gan-Lu-Yin;    Migration;    Restenosis;    Vascular smooth muscle cell;   

DOI  :  10.1186/1472-6882-12-137

 received in 2012-03-20, accepted in 2012-08-17,  发布年份 2012

来源: Springer

【 摘 要 】

BackgroundThis study was to explore the effects of Gan-Lu-Yin (GLY) on the migration of vascular smooth muscle cells (VSMCs) induced by fetal bovine serum and on neointima formation in a rat model of carotid artery balloon injury.MethodsVSMCs were treated with different concentrations of GLY, and then analyzed with Flow cytometric analysis, zymography, transwell, and western blotting. SD rats received balloon-injury were analyzed with H&E staining.ResultsOur results showed that GLY significantly decreased the thickness of neointima. The inhibition by non-cytoxic doses of GLY of VSMCs migration was through its negative regulatory effects on phosphorylated ERK1/2, PI3K/AKT, and FAK. The data showed that GLY can inhibit the migration of VSMCs cells, and might block injury-induced neointima hyperplasia via the inhibition of VSMCs migration, without inducing apoptosis.ConclusionsThese observations provide a mechanism of GLY in attenuating cell migration, thus as a potential intervention for restenosis.

【 授权许可】

Unknown   
© Chien et al.; licensee BioMed Central Ltd. 2012. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.