A Chinese herbal formula

BMC Complementary and Alternative Medicine

Hsu-Chen Cheng²  Po-Liang Cheng²  Ying-Yi Chen¹  Wen-Hsin Lin¹  Chieh-Hsi Wu¹  Ming-Jyh Sheu¹  Yi-Chung Chien² 

[1] School of Pharmacy, China Medical University, 91 Hsueh-Shih Road, Taichung, 404, Taiwan;Department of Life Sciences, and Agricultural Biotechnology Center, National Chung Hsing University, 250 Kuo Kuang Road, Taichung, 402, Taiwan

关键词: Vascular smooth muscle cell;    Restenosis;    Migration;    Gan-Lu-Yin;    Chinese herbs;   

Others  :  1232071
DOI  :  10.1186/1472-6882-12-137

 received in 2012-03-20, accepted in 2012-08-17,  发布年份 2012

【 摘 要 】

Background

This study was to explore the effects of Gan-Lu-Yin (GLY) on the migration of vascular smooth muscle cells (VSMCs) induced by fetal bovine serum and on neointima formation in a rat model of carotid artery balloon injury.

Methods

VSMCs were treated with different concentrations of GLY, and then analyzed with Flow cytometric analysis, zymography, transwell, and western blotting. SD rats received balloon-injury were analyzed with H&E staining.

Results

Our results showed that GLY significantly decreased the thickness of neointima. The inhibition by non-cytoxic doses of GLY of VSMCs migration was through its negative regulatory effects on phosphorylated ERK1/2, PI3K/AKT, and FAK. The data showed that GLY can inhibit the migration of VSMCs cells, and might block injury-induced neointima hyperplasia via the inhibition of VSMCs migration, without inducing apoptosis.

Conclusions

These observations provide a mechanism of GLY in attenuating cell migration, thus as a potential intervention for restenosis.

【 授权许可】

   
2012 Chien et al.; licensee BioMed Central Ltd.

【 图 表 】

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