Chemical composition, antinociceptive, anti-inflammatory and redox properties in vitro of the essential oil from Remirea maritima Aubl. (Cyperaceae)

BMC Complementary and Alternative Medicine

Research Article

José Claudio Fonseca Moreira¹  Daniel Pens Gelain¹  Thallita Kelly Rabelo²  Adriano Antunes Souza Araújo²  Douglas da Silva Prado²  Alessandra Silva Rabelo²  Marília dos Santos Bezerra²  Lucindo José Quintans²  Mairim Russo Serafini²  Marcelia Garcez Dória de Melo²  Emmanoel Vilaça Costa³  Thanany Brasil da Silva³  Paulo Cesar de Lima Nogueira³  Valéria Regina de Souza Moraes³  Ana Paula do Nascimento Prata⁴ 

[1] Centro de Estudos em Estresse Oxidativo, Departamento de Bioquímica, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, 13083-970, Porto Alegre, Rio Grande do Sul, Brazil;Laboratório de Ensaios Farmacêuticos e Toxicidade, Universidade Federal de Sergipe (LeFT/UFS), 49100-000, São Cristóvão, Sergipe, Brazil;Laboratório de Pesquisa em Química Orgânica de Sergipe (LABORGANICS), Departamento de Química, Universidade Federal de Sergipe, 49100-000, São Cristóvão, Sergipe, Brazil;Laboratório de Sistemática Vegetal, Departamento de Biologia, Universidade Federal de Sergipe, 49100-000, São Cristóvão, Sergipe, Brazil;

关键词: Remirea maritima;    Essential oil composition;    Redox properties;   

DOI  :  10.1186/1472-6882-14-514

 received in 2013-11-05, accepted in 2014-12-10,  发布年份 2014

来源: Springer

【 摘 要 】

BackgroundThe present study was carried out to evaluate antioxidant, antinociceptive and anti-inflammatory activities of essential oil from R. maritima (RMO) in experimental protocols.MethodsThe essential oil from the roots and rhizomes of RMO were obtained by hydrodistillation using a Clevenger apparatus, and analyzed by gas chromatography/mass spectrometry (GC/MS). Here, we evaluated free radical scavenging activities and antioxidant potential of RMO using in vitro assays for scavenging activity against hydroxyl radicals, hydrogen peroxide, superoxide radicals, and nitric oxide. The total reactive antioxidant potential (TRAP) and total antioxidant reactivity (TAR) indexes and in vitro lipoperoxidation were also evaluated. The ability of RMO to prevent lipid peroxidation was measured by quantifying thiobarbituric acid-reactive substances (TBARS). NO radical generated at physiological pH was found to be inhibited by RMO, that showed scavenging effect upon SNP-induced NO production at all concentrations. Antinociceptive and anti-inflammatory properties were evaluated by acetic acid writhing reflex, Formalin-induced nociception and Carrageenan-induced edema test.ResultsThe majors compounds identified was remirol (43.2%), cyperene (13.8%), iso-evodionol (5.8%), cyperotundone (5.7%), caryophyllene oxide (4.9%), and rotundene (4.6%). At the TRAP assay, RMO concentration of 1 mg.mL−1 showed anti-oxidant effects and at concentration of 1 and 10 ng.mL−1 RMO showed pro-oxidant effect. RMO at 1 mg.mL−1 also showed significant anti-oxidant capacity in TAR measurement. Concentrations of RMO from 1 ng.mL−1 to 100 μg.mL−1 enhanced the AAPH-induced lipoperoxidation. RMO reduced deoxyribose oxidative damage, induced by the Fenton reaction induction system, at concentrations from 1 ng.mL−1 to 100 μg.mL−1. We observed that RMO caused a significant increase in rate of adrenaline auto-oxidation. On the other hand RMO did not present any scavenging effect in H2O2 formation in vitro. The results of this study revealed that RMO has both peripheral and central analgesic properties. The RMO, all doses, orally (p.o.) administered significantly inhibited (p < 0.05, p < 0.01 and p < 0.001) the acetic acid-induced writhings and two phases of formalin-induced nociception in mice.ConclusionThe RMO demonstrated antioxidant and analgesic profile which may be related to the composition of the oil.

【 授权许可】

Unknown   
© Rabelo et al. 2014. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.