Chemical composition, antinociceptive, anti-inflammatory and redox properties in vitro of the essential oil from Remirea maritima Aubl. (Cyperaceae)

BMC Complementary and Alternative Medicine

Adriano Antunes Souza Araújo⁴  Lucindo José Quintans Jr⁴  Ana Paula do Nascimento Prata³  Valéria Regina de Souza Moraes²  Paulo Cesar de Lima Nogueira²  Emmanoel Vilaça Costa²  Thanany Brasil da Silva²  Marília dos Santos Bezerra⁴  José Claudio Fonseca Moreira¹  Daniel Pens Gelain¹  Douglas da Silva Prado⁴  Marcelia Garcez Dória de Melo⁴  Thallita Kelly Rabelo⁴  Mairim Russo Serafini⁴  Alessandra Silva Rabelo⁴ 

[1] Centro de Estudos em Estresse Oxidativo, Departamento de Bioquímica, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, 13083-970 Porto Alegre, Rio Grande do Sul, Brazil;Laboratório de Pesquisa em Química Orgânica de Sergipe (LABORGANICS), Departamento de Química, Universidade Federal de Sergipe, 49100-000 São Cristóvão, Sergipe, Brazil;Laboratório de Sistemática Vegetal, Departamento de Biologia, Universidade Federal de Sergipe, 49100-000 São Cristóvão, Sergipe, Brazil;Laboratório de Ensaios Farmacêuticos e Toxicidade, Universidade Federal de Sergipe (LeFT/UFS), 49100-000 São Cristóvão, Sergipe, Brazil

关键词: Redox properties;    Essential oil composition;    Remirea maritima;   

Others  :  1229015
DOI  :  10.1186/1472-6882-14-514

 received in 2013-11-05, accepted in 2014-12-10,  发布年份 2014

【 摘 要 】

Background

The present study was carried out to evaluate antioxidant, antinociceptive and anti-inflammatory activities of essential oil from R. maritima (RMO) in experimental protocols.

Methods

The essential oil from the roots and rhizomes of RMO were obtained by hydrodistillation using a Clevenger apparatus, and analyzed by gas chromatography/mass spectrometry (GC/MS). Here, we evaluated free radical scavenging activities and antioxidant potential of RMO using in vitro assays for scavenging activity against hydroxyl radicals, hydrogen peroxide, superoxide radicals, and nitric oxide. The total reactive antioxidant potential (TRAP) and total antioxidant reactivity (TAR) indexes and in vitro lipoperoxidation were also evaluated. The ability of RMO to prevent lipid peroxidation was measured by quantifying thiobarbituric acid-reactive substances (TBARS). NO radical generated at physiological pH was found to be inhibited by RMO, that showed scavenging effect upon SNP-induced NO production at all concentrations. Antinociceptive and anti-inflammatory properties were evaluated by acetic acid writhing reflex, Formalin-induced nociception and Carrageenan-induced edema test.

Results

The majors compounds identified was remirol (43.2%), cyperene (13.8%), iso-evodionol (5.8%), cyperotundone (5.7%), caryophyllene oxide (4.9%), and rotundene (4.6%). At the TRAP assay, RMO concentration of 1 mg.mL⁻¹ showed anti-oxidant effects and at concentration of 1 and 10 ng.mL⁻¹ RMO showed pro-oxidant effect. RMO at 1 mg.mL⁻¹ also showed significant anti-oxidant capacity in TAR measurement. Concentrations of RMO from 1 ng.mL⁻¹ to 100 μg.mL⁻¹ enhanced the AAPH-induced lipoperoxidation. RMO reduced deoxyribose oxidative damage, induced by the Fenton reaction induction system, at concentrations from 1 ng.mL⁻¹ to 100 μg.mL⁻¹. We observed that RMO caused a significant increase in rate of adrenaline auto-oxidation. On the other hand RMO did not present any scavenging effect in H₂O₂ formation in vitro. The results of this study revealed that RMO has both peripheral and central analgesic properties. The RMO, all doses, orally (p.o.) administered significantly inhibited (p < 0.05, p < 0.01 and p < 0.001) the acetic acid-induced writhings and two phases of formalin-induced nociception in mice.

Conclusion

The RMO demonstrated antioxidant and analgesic profile which may be related to the composition of the oil.

【 授权许可】

   
2014 Rabelo et al.; licensee BioMed Central.

【 图 表 】

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