Overexpression of Chromatin Assembly Factor-1/p60 helps to predict the prognosis of melanoma patients

BMC Cancer

Research Article

Massimo Mascolo¹  Maria Luisa Vecchione¹  Loredana Nugnes¹  Maria Siano¹  Gennaro Ilardi¹  Stefania Staibano²  Gaetano De Rosa³  Maria Di Benedetto⁴  Massimiliano Scalvenzi⁵  Guido Molea⁶ 

[1] Department of Biomorphological and Functional Sciences, Pathology Section, University of Naples "Federico II", School of Medicine, Naples, Italy;Department of Biomorphological and Functional Sciences, Pathology Section, University of Naples "Federico II", School of Medicine, Naples, Italy;Cooperative Melanoma Intergroup, University of Naples "Federico II", School of Medicine, Naples, Italy;Department of Biomorphological and Functional Sciences, Pathology Section, University of Naples "Federico II", School of Medicine, Naples, Italy;Cooperative Melanoma Intergroup, University of Naples "Federico II", School of Medicine, Naples, Italy;CROB, Oncology Research Center of Basilicata, Rionero in Vulture, Potenza, Italy;Department of Medicine, University of Naples "Federico II", School of Medicine, Naples, Italy;Department of Systematic Pathology, Section of Dermatology, Allergology and Venereology, University of Naples "Federico II", School of Medicine, Naples, Italy;Cooperative Melanoma Intergroup, University of Naples "Federico II", School of Medicine, Naples, Italy;Department of Systematic Pathology, Section of Plastic Surgery, University of Naples "Federico II", School of Medicine Naples, Italy;Cooperative Melanoma Intergroup, University of Naples "Federico II", School of Medicine, Naples, Italy;

关键词: Melanoma;    Receiver Operating Characteristic;    Cutaneous Melanoma;    Breslow Thickness;    Skin Melanoma;   

DOI  :  10.1186/1471-2407-10-63

 received in 2009-08-06, accepted in 2010-02-24,  发布年份 2010

来源: Springer

【 摘 要 】

BackgroundCutaneous melanoma (CM) is the most lethal form of skin malignancy, which registers a constant increase in incidence worldwide. The identification of molecular alteration(s) involved in its biological aggressiveness represents a major challenge for researchers, considering that existing therapies are ineffective to treat metastasizing cases. The epigenetic control of chromatin dynamics during DNA synthesis, replication, and repair is fundamental for the orderly progression of cell proliferation. The Chromatin Assembly Factor 1 (CAF-1) complex acts as a major regulator of this process; its intermediate (p60) subunit has been recently proposed as a novel proliferation and prognostic marker for several tumors. We aimed to establish if the evaluation of the expression of CAF-1/p60 in primary CM may help define the prevision of outcome of patients.MethodsImmunohistochemistry with anti-CAF-1/p60 was performed on paraffin-embedded tissue sections of 130 cases of primary CM retrieved from the archive files of the Department of Biomorphological and Functional Sciences, Section of Pathology, University "Federico II" of Naples, Italy. Results were compared with histopathological and follow-up data of patients.ResultsCAF-1/p60 was expressed in all CM. A significant statistical association between the overexpression of the protein and the occurrence of skin, node and/or distant metastases (P < 0.05) emerged, independently from histopathological prognostic factors.ConclusionsCAF-1/p60 looks promising as a new prognostic marker for CM and sheds new light on the molecular events associated with photocancerogenesis and melanoma biology.The screening for CAF-1/p60 might contribute to the molecular sub-classification of CM, with improved translational outcomes.

【 授权许可】

Unknown   
© Mascolo et al; licensee BioMed Central Ltd. 2010. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.