Selaginella tamariscina extract suppresses TPA-induced invasion and metastasis through inhibition of MMP-9 in human nasopharyngeal carcinoma HONE-1 cells

BMC Complementary and Alternative Medicine

Research Article

Yi-Hsien Hsieh¹  Chiao-Wen Lin²  Huang-Pin Lin³  Hsin-Yu Ho⁴  Shun-Fa Yang⁵  Mu-Kuan Chen⁶  Buor-Chang Wu⁷  Chung-Han Hsin⁸  Chun-Yi Chuang⁸ 

[1] Department of Biochemistry, School of Medicine, Chung Shan Medical University, Taichung, Taiwan;Department of Dentistry, Chung Shan Medical University Hospital, Taichung, Taiwan;Institute of Oral Sciences, Chung Shan Medical University, 110 Chien-Kuo N. Road, Section 1, Taichung, Taiwan;Department of Otolaryngology, Chung Shan Medical University Hospital, Taichung, Taiwan;Institute of Medicine, Chung Shan Medical University, Taichung, Taiwan;Institute of Medicine, Chung Shan Medical University, Taichung, Taiwan;Department of Medical Research, Chung Shan Medical University Hospital, Taichung, Taiwan;Institute of Medicine, Chung Shan Medical University, Taichung, Taiwan;Department of Otorhinolaryngology-Head and Neck Surgery, Changhua Christian Hospital, Changhua, Taiwan;School of Dentistry, Chung Shan Medical University, Taichung, Taiwan;Department of Dentistry, Chung Shan Medical University Hospital, Taichung, Taiwan;School of Medicine, Chung Shan Medical University, Taichung, Taiwan;Department of Otolaryngology, Chung Shan Medical University Hospital, Taichung, Taiwan;

关键词: Selaginella tamariscina;    MMP-9;    Invasion;    Nasopharyngeal carcinoma;   

DOI  :  10.1186/1472-6882-13-234

 received in 2013-05-15, accepted in 2013-09-19,  发布年份 2013

来源: Springer

【 摘 要 】

BackgroundNasopharyngeal carcinoma (NPC) is known for its high incidence of neck lymph node metastasis, which represents poor prognosis. The present study aimed to examine the anti-metastatic properties of Selaginella tamariscina extract (STE) in human nasopharyngeal carcinoma HONE-1 cells in vitro.MethodsCell viability was examined by MTT assay, whereas cell motility was measured by invasive, migration and would healing assays. Real-time PCR, and promoter assays confirmed the inhibitory effects of STE on matrix metalloproteinase-9 (MMP-9) mRNA level in HONE-1 cells.ResultsThe STE inhibits 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced HONE-1 cell migration and invasion in a concentration-dependent manner. By zymographic and Western blot analyses, STE was shown to inhibit the activities and expression of MMP-9. Treatment of STE on TPA-induced HONE-1 cells inhibited MMP-9 expression and ERK1/2 phosphorylation without affecting JNK and p38 phosphorylation.ConclusionsSTE inhibits MMP-9 expression and HONE-1 cell metastasis. Its inhibitory effects may involve the Src/FAK/ERK 1/2 pathway. STE may have the potential of being an anti-metastatic agent against NPC.

【 授权许可】

Unknown   
© Hsin et al.; licensee BioMed Central Ltd. 2013. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.