Screening and evaluating of long noncoding RNAs in the puberty of goats

BMC Genomics

Research Article

Lei Luo¹  Kaifa Zhang¹  Chen Yang¹  Xiaoxiao Gao¹  Xiumei Li²  Hongguo Cao²  Jianping Ding²  Xiaorong Zhang²  Fugui Fang²  Jing Ye²  Yunsheng Li²  Ya Liu²  Yunhai Zhang²  Yinghui Ling² 

[1] Anhui Provincial Laboratory of Animal Genetic Resources Protection and Breeding, College of Animal Science and Technology, Anhui Agricultural University, 130 Changjiang West Road, 230036, Hefei, Anhui, China;Anhui Provincial Laboratory of Animal Genetic Resources Protection and Breeding, College of Animal Science and Technology, Anhui Agricultural University, 130 Changjiang West Road, 230036, Hefei, Anhui, China;Anhui Provincial Laboratory for Local Livestock and Poultry Genetic Resource Conservation and Bio-Breeding, 130 Changjiang West Road, 230036, Hefei, Anhui, China;Department of Animal Veterinary Science, College of Animal Science and Technology, Anhui Agricultural University, 130 Changjiang West Road, 230036, Hefei, Anhui, China;

关键词: LncRNA;    Puberty;    Goat;    Hypothalamus;    Transcriptome;   

DOI  :  10.1186/s12864-017-3578-9

 received in 2016-07-13, accepted in 2017-02-09,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundLong noncoding RNAs (lncRNAs) are involved in regulating animal development, however, their function in the onset of puberty in goats remain largely unexplored. To identify the genes controlling the regulation of puberty in goats, we measured lncRNA and mRNA expression levels from the hypothalamus.ResultsWe applied RNA sequencing analysis to examine the hypothalamus of pubertal (case; n = 3) and prepubertal (control; n = 3) goats. Our results showed 2943 predicted lncRNAs, including 2012 differentially expressed lncRNAs, which corresponded to 5412 target genes. We also investigated the role of lncRNAs that act cis and trans to the target genes and found a number of lncRNAs involved in the regulation of puberty and reproduction, as well as several pathways related to these processes. For example, oxytocin signaling pathway, sterol biosynthetic process, and pheromone receptor activity signaling pathway were enriched as Kyoto Encyclopedia of Genes and Genomes (KEGG) or gene ontology (GO) analyses showed.ConclusionOur results clearly demonstrate that lncRNAs play an important role in regulating puberty in goats. However, further research is needed to explore the functions of lncRNAs and their predicted targets to provide a detailed expression profile of lncRNAs on goat puberty.

【 授权许可】

CC BY   
© The Author(s). 2017