Polar extracts from (Tunisian) Acacia salicina Lindl. Study of the antimicrobial and antigenotoxic activities

BMC Complementary and Alternative Medicine

Research Article

Jihed Boubaker¹  Hedi Ben Mansour¹  Leila Chekir Ghedira²  Kamel Ghedira³ 

[1] Laboratory of Cellular and Molecular Biology, Faculty of Dental Medicine, University of Monastir, 5000, Rue Avicenne, Monastir, Tunisia;Laboratory of Cellular and Molecular Biology, Faculty of Dental Medicine, University of Monastir, 5000, Rue Avicenne, Monastir, Tunisia;Department of Cellular and Molecular Biology, Faculty of Dental Medicine, 5000, Rue Avicenne, Monastir, Tunisia;Unity of Pharmacognosy/Molecular Biology, Faculty of Pharmacy, University of Monastir, 5000, Rue Avicenne, Monastir, Tunisia;

关键词: Acacia salicina;    Antigenotoxic activity;    Antioxidant activities;    Ames assay;   

DOI  :  10.1186/1472-6882-12-37

 received in 2011-09-03, accepted in 2012-04-10,  发布年份 2012

来源: Springer

【 摘 要 】

BackgroundMethanolic, aqueous and Total Oligomer Flavonoids (TOF)-enriched extracts obtained from the leaves of Acacia salicina 'Lindl.' were investigated for antibacterial, antimutagenic and antioxidant activities.MethodsThe antimicrobial activity was tested on the Gram positive and Gram negative reference bacterial strains. The Mutagenic and antimutagenic activities against direct acting mutagens, methylmethane sulfonate (MMS) and 4-nitro-o-phenylenediamine (NOPD), and indirect acting mutagens, 2-aminoanthracene (2-AA) and benzo[a]pyrene (B(a)P) were performed with S. typhimurium TA102 and TA98 assay systems. In addition, the enzymatic and nonenzymatic methods were employed to evaluate the anti-oxidative effects of the tested extracts.ResultsA significant effect against the Gram positive and Gram negative reference bacterial strains was observed with all the extracts. The mutagenic and antimutagenic studies revealed that all the extracts decreased the mutagenicity induced by B(a)P (7.5 μg/plate), 2-AA (5 μg/plate), MMS (1.3 mg/plate) and NOPD (10 μg/plate). Likewise, all the extracts showed an important free radical scavenging activity towards the superoxide anion generated by the xanthine/xanthine oxidase assay system, as well as high Trolox Equivalent Antioxidant Capacity (TEAC), against the 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS)+• radical. TOF-enriched extract exhibited the highest protective effect against free radicals, direct acting-mutagen and metabolically activated S9-dependent mutagens.ConclusionsThe present study indicates that the extracts from A. salicina leaves are a significant source of compounds with the antimutagenic and antioxidant activities, and this may be useful for developing potential chemopreventive substances.

【 授权许可】

Unknown   
© Boubaker et al; licensee BioMed Central Ltd. 2012. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.