期刊论文详细信息
BMC Complementary and Alternative Medicine
Polar extracts from (Tunisian) Acacia salicina Lindl. Study of the antimicrobial and antigenotoxic activities
Leila Chekir Ghedira2  Kamel Ghedira1  Hedi Ben Mansour3  Jihed Boubaker3 
[1] Unity of Pharmacognosy/Molecular Biology, Faculty of Pharmacy, University of Monastir, Rue Avicenne, Monastir 5000, Tunisia;Department of Cellular and Molecular Biology, Faculty of Dental Medicine, Rue Avicenne, 5000 Monastir, Tunisia;Laboratory of Cellular and Molecular Biology, Faculty of Dental Medicine, University of Monastir, Rue Avicenne, Monastir 5000, Tunisia
关键词: Ames assay;    Antioxidant activities;    Antigenotoxic activity;    Acacia salicina;   
Others  :  1232718
DOI  :  10.1186/1472-6882-12-37
 received in 2011-09-03, accepted in 2012-04-10,  发布年份 2012
PDF
【 摘 要 】

Background

Methanolic, aqueous and Total Oligomer Flavonoids (TOF)-enriched extracts obtained from the leaves of Acacia salicina 'Lindl.' were investigated for antibacterial, antimutagenic and antioxidant activities.

Methods

The antimicrobial activity was tested on the Gram positive and Gram negative reference bacterial strains. The Mutagenic and antimutagenic activities against direct acting mutagens, methylmethane sulfonate (MMS) and 4-nitro-o-phenylenediamine (NOPD), and indirect acting mutagens, 2-aminoanthracene (2-AA) and benzo[a]pyrene (B(a)P) were performed with S. typhimurium TA102 and TA98 assay systems. In addition, the enzymatic and nonenzymatic methods were employed to evaluate the anti-oxidative effects of the tested extracts.

Results

A significant effect against the Gram positive and Gram negative reference bacterial strains was observed with all the extracts. The mutagenic and antimutagenic studies revealed that all the extracts decreased the mutagenicity induced by B(a)P (7.5 μg/plate), 2-AA (5 μg/plate), MMS (1.3 mg/plate) and NOPD (10 μg/plate). Likewise, all the extracts showed an important free radical scavenging activity towards the superoxide anion generated by the xanthine/xanthine oxidase assay system, as well as high Trolox Equivalent Antioxidant Capacity (TEAC), against the 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS)+• radical. TOF-enriched extract exhibited the highest protective effect against free radicals, direct acting-mutagen and metabolically activated S9-dependent mutagens.

Conclusions

The present study indicates that the extracts from A. salicina leaves are a significant source of compounds with the antimutagenic and antioxidant activities, and this may be useful for developing potential chemopreventive substances.

【 授权许可】

   
2012 Boubaker et al; licensee BioMed Central Ltd.

【 预 览 】
附件列表
Files Size Format View
20151116030304339.pdf 315KB PDF download
Figure 1. 41KB Image download
【 图 表 】

Figure 1.

【 参考文献 】
  • [1]Swayamjot K, Husheem M, Saroj A, Pirkko LH, Subodh-Kumar K: The in vitro cytotoxic and apoptotic activity of Triphala-an Indian herbal drug. J Ethnopharmacol 2005, 97:15-20.
  • [2]Wadood A, Wadood N, Wahid-Shah SA: Effects of Acacia arabica and Caralluma edulis on blood glucose levels of normal and alloxan diabetic rabbits. J Pak Med Assoc 1989, 39:208-212.
  • [3]Sotohy SA, Sayed AN, Ahmed MM: Effect of tannin-rich plant (Accacia nilotica) on some nutritional and bacteriological parameters in goats. Deutsche Tierarztliche Wochenschrift 1997, 104:432-435.
  • [4]Dafallah AA, Al-Mustapha Z: Investigation of the anti-inflammatory activity of Acacia nilotica and Hibiscus sabdariffa. Am J Chinese Med 1996, 24:263-269.
  • [5]Ghosh NK, Babu SP, Sukul NC, Ito A: Cestocidal activity of Acacia auriculiformis. J of Helmintol 1996, 70:171-172.
  • [6]Amos S, Akah PA, Odukwe CJ, Gamaniel KS, Wambede C: The pharmacological effects of an aqueous extract from Acacia nilotica seeds. Phytother Res 1999, 13:683-685.
  • [7]Gilani AH, Shaheen F, Zaman M, Janbaz KH, Shah BH, Akhtar MS: Studies on hypertensive and antispasmodic activities of methanol extract of Acacia nilotica pods. Phytother Res 1999, 14:510-516.
  • [8]Shah BH, Safdar B, Virani SS, Nawaz Z, Saeed SA, Gilani AH: The antiplatelet aggregatory activityof Accacia nilotica is due to blockage of calcium influx through membrane calcium channels. General Pharmacol 1997, 29:251-255.
  • [9]Hussein G, Miyashiro H, Nakamura N, Hattori M, Kakiuchi N, Shimotohno K: Inhibitory effects of Sudanese medicinale plant eextracts on hepatitis C virus (HCV). Phytother Res 2000, 14:510-516.
  • [10]Bouhlel I, Valenti K, Kilani S, Skandrani I, Sghaier MB: Antimutagenic, antigenotoxic and antioxidant activities of Acacia salicina extracts (ASE) and modulation of cell gene expression by H2O2 and ASE treatment. TIV 2008, 2008(22):1264-1272.
  • [11]Getachew G, Makkar HP, Becker : Tannins in tropical browses: effects on in vitro microbial fermentation and microbial protein synthesis in media containing different amounts of nitrogen. J Agric Food Chem 2000, 48:3581-3588.
  • [12]Ben-Mansour H, Boubaker J, Bouhlel I, Mahmoud A, Bernillon S, Ben-Chibani J, Ghedira K, Chekir-Ghedira L: Antigenotoxic activities of crude extracts from Acacia salicina leaves. Environ Mol Mutagen 2007, 48:58-66.
  • [13]Chiang LC, Chiang W, Liu MC, Lin CC: In vitro antiviral activities of Caesalpinia pulcherrima and its related flavonoids. J Antimicrob Chemother 2003, 52:194-198.
  • [14]Mead PS, Slutsker L, Dietz V, McCaig LF, Bresee JS, Shapiro C, Griffin PM, Tauxe RV: Food-related illness and death in the United States. Emerg Infect Dis 1999, 5:607-625.
  • [15]O'Brien SJ, de Valk H: Salmonella-"old" organism, continued challenges! Eurosurveillance 2003, 8:29-31.
  • [16]Dhidah M, Trabelsi A, Mazoughi R, Dhidah L, Boujaafar N, Jeddi M: Salmonella and salmonelloses in the region of Sousse (Tunisia). Microbiol Hyg Alim 1995, 19:3-7.
  • [17]Van der Berg R, Haenen GRMM, Van der Berg H, Van den Vijgh W, Bast A: The predictive value of the antioxidant capacity of structurally related flavonoids using the Trolox equivalent antioxidant capacity (TEAC) assay. Food Chem 2000, 70:391-395.
  • [18]Villaño D, Fernãndez-Pachõn MS, Troncoso AM, Garciã-Parrilla MC: The antioxidant activity of wines determined by the ABTS+• method: influence of sample dilution and time. Talanta 2004, 64:501-509.
  • [19]Ames BN, McCann J, Yamasaki E: Methods for detecting carcinogens and mutagens with the Salmonella/mammalian microsome mutagenicity test. Mut Res 1975, 31:347-364.
  • [20]Marques RCP, de Medeiros SRB, da Silva Dias C, Barbosa-Filho JM, Agnez-Lima LF: Evaluation of the mutagenic potential of yangambin and of the hydroalcoholic extract of Ocotea duckei by the Ames test. Mutat Res 2003, 536:117-120.
  • [21]Teel RW: Ellargic acid binding to DNA as a possible mechanism for its antimutagenic and anticarcinogenic action. Cancer Letter 1986, 30:329-336.
  • [22]Boubaker J, Skandrani I, Bouhlel I, Ben Sghaier M, Neffati A, Ghedira K, Chekir-Ghedira L: Mutagenic, antimutagenic and antioxidant potency of leaf extracts from Nitraria retusa. Food and Chem Toxicol 2010, 48:2283-2290.
  • [23]Vaya J, Mohmod S, Goldblum A, Aviram M, Volkavor N, Shaalam A, Musa R, Tamir S: Inhibition of LDL oxidation by flavonoïds in relation to their structure and calculated enthalpy. Phytochem 2003, 62:89-99.
  • [24]Zoran M, Dorde M, Nada K: Polyphenol contents and antioxidant activity of Maydis stigma extracts. Biores. Technol 2005, 96:873-877.
  • [25]Edenharder R, Grunhage D: Free radical scavenging abilities of flavonoids as mechanism of protection against mutagenicity induced by test-butyl hydroperoxide or cumene hydroperoxide in Salmonella typhimurium TA 102. Mutat Res 2003, 540:1-18.
  • [26]Levin DE, Hollstein M, Christman MF, Schwiers EA, Ames BN: A new Salmonella tester strain (TA102) with A.T base pairs at the site of mutation detects oxidative mutagens. Proc Natl Acad Sci USA 1982, 79:7445-7449.
  • [27]Harris CC, Vähäkangas K, Newman MJ, Trivers GE, Shamsuddin A, Sinopoli N, Mann DL, Wright WE: Detection of benzo[a]pyrene diol epoxide-DNA adducts in peripheral blood lymphocytes and antibodies to the adducts in serum from coke oven workers. Proc Natl Acad Sci USA 1985, 82:6672-6676.
  • [28]Horn RC, Ferrao VVM: Antimutagenic activity of extracts of natural substances in the Salmonella microsome assay. Mutag 2003, 18:113-118.
  • [29]Bouhlel I, Ben Mansour H, Limem I, Ben Sghaier M, Mahmoud A, Ben Chibani J, Ghedira K, Chekir-Ghedira L: Screening of antimutagenicity via antioxidant activity in different extracts from the leaves of Acacia salicina from the center of Tunisia. Environm Toxicol Pharmacol 2007, 23:56-63.
  • [30]Quillardet PQ, Hofnung M: The SOS Chromotest colorimetric bacterial assay for genotoxins: Procedures. Mutat Res 1985, 147:65-78.
  • [31]Chaib M, Boukhris M: Flore succinct et illustré des zones arides et sahariennes de Tunisie. 1998, 43-44.
  • [32]Maron DM, Ames BN: Revised methods for the Salmonella mutagenicity test. Mutat Res 1983, 113:173-215.
  • [33]Hartman PE, Ames BN, Roth JR, Branes WN, Levin DE: Target sequences for mutagenesis in Salmonella histidine-requiring mutants. Environ Mol Mutagen 1986, 8:631-641.
  • [34]Koch WH, Henrikson EN, Cebula TA: Molecular analyses of Salmonella hisG428 ochre revertants for rapid characterization of mutational specificity. Mutagenesis 1996, 11:341-348.
  • [35]Hayder N, Abdelwahed A, Kilani S, Ammar RB, Mahmoud A, Ghedira K, ChekirGhedira L: Anti-genotoxic and free-radical scavenging activities of extracts from (Tunisian) Myrtus communis. Mut Res 2004, 564:89-95.
  • [36]Cremieux A, Fleurette J: Methods of testing disinfectants. In disinfection, sterilization and preservation. 4th edition. Lea and Febiger, Philadelphia and London; 1991:1009-1027.
  • [37]Ben Ammar R, Kilani S, Bouhlel I, Skandrani I, Naffeti A, Boubaker J, Ben Sghaier M, Bhouri W, Mahmoud A, Chekir L, Ghedira K: Antibacterial and cytotoxic activities of extracts from (Tunisian) Rhamnus alaternus (Rhamnaceae). Ann Microbio 2007, 57:453-460.
  • [38]Re P, Proteggente R, Pannala N, Yang A, Rice-Evans CMA: Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic Biol Med 1999, 26:1231-1237.
  • [39]Oyangagui Y: Reevaluation of assay methods and establishment of kit for superoxide dimutase activity. Analy Biochem 1984, 142:290-296.
  • [40]Hu JP, Calomme M, Lasure A, De Bruyne T, Pieters L, Vlietinck A, Vanden Berghe DA: Structure activity relationship of flavonoids with superoxide scavenging activity. Biol. Trace Elem Res 1995, 47:327-331.
  • [41]Russo A, Cardile V, Lombardo L, Vanella L, Vanella A, Garbarino JA: Antioxidant and antiproliferative action of methanolic extract of Geum Quellyon sweet roots in human tumor cell lines. Journal of Ethnopharma 2005, 100:323-332.
  • [42]Bradford MM: A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye Binding. Annals Biochemestry 1976, 72:248-254.
  文献评价指标  
  下载次数:5次 浏览次数:5次