The use of DNA from archival dried blood spots with the Infinium HumanMethylation450 array

BMC Biotechnology

Research Article

Gianluca Severi¹  Dallas R English¹  Laura Baglietto¹  Graham G Giles¹  Nicholas C Wong²  John L Hopper³  JiHoon E Joo⁴  Melissa C Southey⁴  Helen Tsimiklis⁴  Ee Ming Wong⁴  Daniel J Park⁴  Chol-Hee Jung⁵ 

[1] Cancer Epidemiology Centre, Cancer Council Victoria, 3053, Victoria, Australia;Centre for Molecular, Environmental, Genetic and Analytic Epidemiology, The University of Melbourne, 3010, Victoria, Australia;Cancer and Disease Epigenetics, Murdoch Childrens Research Institute, Department of Paediatrics, The University of Melbourne, Royal Children’s Hospital, 3052, Victoria, Australia;Centre for Molecular, Environmental, Genetic and Analytic Epidemiology, The University of Melbourne, 3010, Victoria, Australia;Genetic Epidemiology Laboratory, Department of Pathology, The University of Melbourne, 3010, Victoria, Australia;Life Sciences Computation Centre, Victorian Life Sciences Computation Initiative Carlton, 3010, Victoria, Australia;

关键词: DNA methylation;    Epigenetics;    Infinium HumanMethylation450;    Dried blood spots;    Guthrie cards;    Genome-wide DNA methylation;   

DOI  :  10.1186/1472-6750-13-23

 received in 2012-11-16, accepted in 2013-03-01,  发布年份 2013

来源: Springer

【 摘 要 】

BackgroundDried blood (Guthrie card) spots provide an efficient way to collect and store blood specimens. DNA from this source has been utilised for a number of molecular analyses including genome-wide association studies, but only few studies have tested the feasibility of using it for epigenetic applications, particularly at a genome-wide level.ResultsIn this study, we demonstrate the successful use of DNA isolated from archived dried blood spots for the Infinium HumanMethylation450 Beadchip, along with DNA from matched frozen buffy coats. We obtained high quality and reproducible genome-wide DNA methylation profiles using both sample types. We also report high correlations (r > 0.9907) between DNA obtained from matched dried blood spots and frozen buffy coats, sufficient to distinguish between unrelated individuals.ConclusionsWe, thus, demonstrate that DNA from archived dried blood spots is suitable for genome-wide DNA methylation profiling.

【 授权许可】

Unknown   
© Joo et al.; licensee BioMed Central Ltd. 2013. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.