Continuous enhancement of iturin A production by Bacillus subtilis with a stepwise two-stage glucose feeding strategy

BMC Biotechnology

Research Article

Mian Guo¹  Hu Jin¹  Yanxing Niu¹  Shouwen Chen¹  Chuanjiong Hu¹  Kunpeng Li¹  Fenghong Huang² 

[1] Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, No. 2 Xudong Second Road, 430062, Wuhan, China;Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, No. 2 Xudong Second Road, 430062, Wuhan, China;Hubei Key Laboratory of Lipid Chemistry and Nutrition, 430062, Wuhan, China;

关键词: Bacillus subtilis;    Iturin A;    Lipopeptide;    Rapeseed meal;    Fed-batch fermentation;   

DOI  :  10.1186/s12896-015-0172-6

 received in 2014-08-21, accepted in 2015-05-21,  发布年份 2015

来源: Springer

【 摘 要 】

BackgroundThe lipopeptide antibiotic iturin A is an attractive biopesticide with the potential to replace chemical-based pesticides for controlling plant pathogens. However, its industrial fermentation has not been realized due to the high production costs and low product concentrations. This study aims to enhance iturin A production by performing a novel fermentation process with effective glucose feeding control using rapeseed meal as a low-cost nitrogen source.ResultsWe demonstrated that continuous and significant enhancement of iturin A production could be achieved by a novel two-stage glucose-feeding strategy with a stepwise decrease in feeding rate. Using this strategy, the ratio of spores to total cells could be maintained at a desirable/stable level of 0.80–0.86, and the reducing sugar concentration could be controlled at a low level of 2–3 g/L so that optimal substrate balance could be maintained throughout the feeding phase. As a result, the maximum iturin A concentration reached 1.12 g/L, which was two-fold higher than that of batch culture.ConclusionsThis is the first report which uses control of the glucose supply to improve iturin A production by fed-batch fermentation and identifies some important factors necessary to realize industrial iturin A production. This approach may also enhance the production of other useful secondary metabolites by Bacillus subtilis.

【 授权许可】

Unknown   
© Jin et al. 2015. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.