Continuous enhancement of iturin A production by Bacillus subtilis with a stepwise two-stage glucose feeding strategy

BMC Biotechnology

Hu Jin²  Kunpeng Li²  Yanxing Niu²  Mian Guo²  Chuanjiong Hu²  Shouwen Chen²  Fenghong Huang¹ 

[1] Hubei Key Laboratory of Lipid Chemistry and Nutrition, Wuhan 430062, China

[2] Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, No. 2 Xudong Second Road, Wuhan 430062, China

关键词: Fed-batch fermentation;    Rapeseed meal;    Lipopeptide;    Iturin A;    Bacillus subtilis;   

Others  :  1216692
DOI  :  10.1186/s12896-015-0172-6

 received in 2014-08-21, accepted in 2015-05-21,  发布年份 2015

【 摘 要 】

Background

The lipopeptide antibiotic iturin A is an attractive biopesticide with the potential to replace chemical-based pesticides for controlling plant pathogens. However, its industrial fermentation has not been realized due to the high production costs and low product concentrations. This study aims to enhance iturin A production by performing a novel fermentation process with effective glucose feeding control using rapeseed meal as a low-cost nitrogen source.

Results

We demonstrated that continuous and significant enhancement of iturin A production could be achieved by a novel two-stage glucose-feeding strategy with a stepwise decrease in feeding rate. Using this strategy, the ratio of spores to total cells could be maintained at a desirable/stable level of 0.80–0.86, and the reducing sugar concentration could be controlled at a low level of 2–3 g/L so that optimal substrate balance could be maintained throughout the feeding phase. As a result, the maximum iturin A concentration reached 1.12 g/L, which was two-fold higher than that of batch culture.

Conclusions

This is the first report which uses control of the glucose supply to improve iturin A production by fed-batch fermentation and identifies some important factors necessary to realize industrial iturin A production. This approach may also enhance the production of other useful secondary metabolites by Bacillus subtilis.

【 授权许可】

   
2015 Jin et al.

【 图 表 】

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