Detailed phenotyping identifies genes with pleiotropic effects on body composition

BMC Genomics

Research Article

Sunduimijid Bolormaa¹  Ben J. Hayes²  Hans D. Daetwyler²  Michael E. Goddard³  Julius H.J. van der Werf⁴  David Pethick⁵ 

[1] AgriBio, Centre for AgriBioscience, Department of Economic Development, Jobs, Transport and Resources, 3083, Bundoora, VIC, Australia;Cooperative Research Centre for Sheep Industry Innovation, 2351, Armidale, NSW, Australia;AgriBio, Centre for AgriBioscience, Department of Economic Development, Jobs, Transport and Resources, 3083, Bundoora, VIC, Australia;School of Applied Systems Biology, La Trobe University, 3083, Bundoora, VIC, Australia;Cooperative Research Centre for Sheep Industry Innovation, 2351, Armidale, NSW, Australia;AgriBio, Centre for AgriBioscience, Department of Economic Development, Jobs, Transport and Resources, 3083, Bundoora, VIC, Australia;School of Land and Environment, University of Melbourne, 3010, Parkville, VIC, Australia;School of Environmental and Rural Science, University of New England, 2351, Armidale, NSW, Australia;Cooperative Research Centre for Sheep Industry Innovation, 2351, Armidale, NSW, Australia;School of Veterinary and Life Sciences, Murdoch University, 6150, Murdoch, WA, Australia;Cooperative Research Centre for Sheep Industry Innovation, 2351, Armidale, NSW, Australia;

关键词: GWAS;    Multi-trait;    Meta-analysis;    Pleiotropy;    Genes;    Body composition;    Sheep;    Human;   

DOI  :  10.1186/s12864-016-2538-0

 received in 2015-09-23, accepted in 2016-02-05,  发布年份 2016

来源: Springer

【 摘 要 】

BackgroundGenetic variation in both the composition and distribution of fat and muscle in the body is important to human health as well as the healthiness and value of meat from cattle and sheep. Here we use detailed phenotyping and a multi-trait approach to identify genes explaining variation in body composition traits.ResultsA multi-trait genome wide association analysis of 56 carcass composition traits measured on 10,613 sheep with imputed and real genotypes on 510,174 SNPs was performed. We clustered 71 significant SNPs into five groups based on their pleiotropic effects across the 56 traits. Among these 71 significant SNPs, one group of 11 SNPs affected the fatty acid profile of the muscle and were close to 8 genes involved in fatty acid or triglyceride synthesis. Another group of 23 SNPs had an effect on mature size, based on their pattern of effects across traits, but the genes near this group of SNPs did not share any obvious function. Many of the likely candidate genes near SNPs with significant pleiotropic effects on the 56 traits are involved in intra-cellular signalling pathways. Among the significant SNPs were some with a convincing candidate gene due to the function of the gene (e.g. glycogen synthase affecting glycogen concentration) or because the same gene was associated with similar traits in other species.ConclusionsUsing a multi-trait analysis increased the power to detect associations between SNP and body composition traits compared with the single trait analyses. Detailed phenotypic information helped to identify a convincing candidate in some cases as did information from other species.

【 授权许可】

CC BY   
© Bolormaa et al. 2016

【 图 表 】

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