Essential oils: in vitro activity against Leishmania amazonensis, cytotoxicity and chemical composition

BMC Complementary and Alternative Medicine

Research Article

Maria Lucília dos Santos¹  Izabela M. D. Bastos²  Camila Lasse Silva²  Jaime Martins de Santana²  Clênia dos Santos Azevedo²  Flávia Nader Motta³  Milene Aparecida Andrade³ 

[1] Institute of Chemistry, The University of Brasilia, Brasília, Brazil;Pathogen-Host Interface Laboratory, Department of Cell Biology, The University of Brasília, Campus Darcy Ribeiro, Bloco I, CEP 70910-900, Brasília, DF, Brazil;Pathogen-Host Interface Laboratory, Department of Cell Biology, The University of Brasília, Campus Darcy Ribeiro, Bloco I, CEP 70910-900, Brasília, DF, Brazil;Faculty of Ceilândia, The University of Brasília, Brasília, Brazil;

关键词: Secondary metabolites;    Leishmania;    Natural products;    Ferula galbaniflua;   

DOI  :  10.1186/s12906-016-1401-9

 received in 2016-04-20, accepted in 2016-10-13,  发布年份 2016

来源: Springer

【 摘 要 】

BackgroundThe current chemotherapy for cutaneous leishmaniosis (CL) has a series of drug limitations such as toxic side effects, long duration, high costs and drug resistance, which requires the development of new drugs or effective alternatives to the CL treatment. Essential oils (EOs) are complex mixtures of secondary metabolites from various plants. It has been shown that several EOs, or their constituents, have inhibitory activity against protozoa. Thus, this study aims to evaluate the biological activity of different essential oils (EOs) on Leishmania (L.) amazonensis promastigotes forms, as well as their cytotoxicity on mammalian cells and chemical composition.MethodsSixteen EOs were evaluated by mean of IC50/24 h and cytotoxicity against L6 cells (CC50/24 h) using Resazurin assay. Only those EOs that presented better results for IC50/24 h were submitted to GC–MS analysis to determine their chemical constitution.ResultsThe EO from Cinnamodendron dinisii, Matricaria chamomilla, Myroxylon peruiferum, Salvia sclarea, Bulnesia sarmientoi, Ferula galbaniflua, Siparuna guianensis and Melissa officinalis were the most active against L. amazonensis with IC50/24 h ranging from 54.05 to 162.25 μg/mL. Analysis of EOs by GC–MS showed mainly the presence of β-farnesene (52.73 %) and bisabolol oxide (12.09 %) for M. chamomilla; α-copaene (13.41 %), safrole (8.35 %) and δ-cadinene (7.08 %) for M. peruiferum; linalool (28.80 %) and linalyl acetate (60.08 %) for S. sclarea; guaiol (48.29 %) and 2-undecanone (19.49 %) for B. sarmientoi; ethyl phthalate (13.09 %) and methyl-8-pimaren-18-oate (41.82 %) for F. galbaniflua; and neral (37.18 %) and citral (5.02 %) for M. officinalis.ConclusionThe EO from F. galbaniflua showed to be effective against L. amazonensis promastigotes forms and presented low cytotoxic activity against L6 cells. Thus, it represents a strong candidate for future studies aiming its molecular activity on these pathogenic parasites.

【 授权许可】

CC BY   
© The Author(s). 2016