Genistein treatment improves fracture resistance in obese diabetic mice

BMC Endocrine Disorders

Research Article

Britton Odle¹  Nathan Dennison¹  Jeffrey H. Plochocki²  Layla Al-Nakkash³  Tom L. Broderick⁴ 

[1] Arizona College of Osteopathic Medicine, Midwestern University, Glendale, AZ, USA;Department of Anatomy, Arizona College of Osteopathic Medicine, Midwestern University, 85308, Glendale, AZ, USA;Department of Physiology, Arizona College of Osteopathic Medicine, Midwestern University, Glendale, AZ, USA;Department of Physiology, Laboratory of Diabetes and Exercise Metabolism, Arizona College of Osteopathic Medicine, Midwestern University, Glendale, AZ, USA;

关键词: Fracture Resistance;    Benzoyl Peroxide;    Obese Mouse;    Femur Length;    Limb Bone;   

DOI  :  10.1186/s12902-016-0144-4

 received in 2016-06-25, accepted in 2016-10-27,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundObese, type two diabetics are at an increased risk for fracturing their limb bones in comparison to the general population. Phytoestrogens like as the soy isoflavone genistein have been shown to protect against bone loss. In this study, we tested the effects of genistein treatment on femurs of ob/ob mice, a model for obesity and type two diabetes mellitus.MethodsTwenty six-week-old female mice were divided into obese (ob/ob) control, obese genistein-treated, lean (ob/+) control, and lean genistein-treated groups (n = 5 each). Treatment with genistein consisted of 600 mg genistein/kg diet. Control mice were given standard rodent chow. At the end of a four-week treatment period, bone histomorphometric and three-point bending properties were compared among groups.ResultsObese mice had larger bone areas (B.Ar.; P < 0.05) and total areas (Tt.Ar.; P < 0.05), but similar bone volume (B.Ar./Tt.Ar.; P > 0.05) of the proximal femoral epiphysis in comparison to lean mice. Treatment with genistein decreased Tt.Ar. and femur length, and increased ultimate force required to fracture the femur and the maximum deformation to failure (P < 0.05).ConclusionsGenistein improves resistance to fracture from bending loads.

【 授权许可】

CC BY   
© The Author(s). 2017