Nam Dia long, a Vietnamese folk formula, induces apoptosis in MCF-7 cells through various mechanisms of action

BMC Complementary and Alternative Medicine

Research Article

Thuy-Duong Ho-Huynh¹  My-Nuong Thi Nguyen² 

[1] Department of Genetics, Faculty of Biology and Biotechnology, University of Science, VNU-HCM, 227 Nguyen Van Cu Street, Ward 4, District 5, Ho Chi Minh City, Vietnam;Molecular Genetics and Genomics Research Group, Cancer Research Laboratory, University of Science, VNU-HCM, Building B6.1, Dong Hoa Village, Di An District, Binh Duong Province, Vietnam;

关键词: Traditional medicine;    Breast cancer;    Apoptosis;    Microarray profiling;    Nam Dia long;   

DOI  :  10.1186/s12906-017-2027-2

 received in 2017-08-23, accepted in 2017-11-24,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundThe holistic approach of traditional medicine renders the identification of its mechanisms of action difficult. Microarray technology provides an efficient way to analyze the complex genome-wide gene expression of cells treated with mixtures of medicinal ingredients. We performed transcriptional profiling of MCF-7 cells treated with Nam Dia Long (NDL), a Vietnamese traditional formula, to explore the mechanism of action underlying the apoptosis inducing effect of this formula reported in a previous study.MethodsMCF-7 cells were treated with aqueous extracts of NDL at the IC50 concentration for 24, 36 and 48 h. Total RNAs at 24 h and 48 h were subsequently extracted, reverse transcribed and submitted to microarray expression profiling using the Human HT-12 v4.0 Expression Bead Chip (Illumina). Functional analyses were performed using the Database for Annotation, Visualization and Integrated Discovery and the Ingenuity Pathways Analysis. The expression level from selected genes at the three time points were assessed by quantitative real-time RT-PCR and Western blot.ResultsFifty-four and 601 genes were differentially expressed at 24 and 48 h of NDL treatment, respectively. Genes with altered expression at 24 h were mostly involved in cell responses to xenobiotic stress whereas genes differentially expressed at 48 h were related to endoplasmic reticulum stress, DNA damage and cell cycle control. Apoptosis of NDL treated MCF-7 cells resulted from a combination of different mechanisms including the intrinsic and extrinsic pathways, cell cycle arrest- and oxidative stress-related cell death.ConclusionNDL elicited a two-stage response in MCF-7 treated cells with apoptosis as the ultimate result. The various mechanisms inducing apoptosis reflected the complexity of the formula composition.

【 授权许可】

CC BY   
© The Author(s). 2017