Early transcriptomic response to Fe supply in Fe-deficient tomato plants is strongly influenced by the nature of the chelating agent

BMC Genomics

Research Article

Laura Zanin¹  Nicola Tomasi¹  Roberto Pinton¹  Anita Zamboni²  Zeno Varanini²  Linda Avesani²  Stefano Cesco³ 

[1] Department of Agriculture and Environmental Sciences, University of Udine, via delle Scienze 208, 33100, Udine, Italy;Department of Biotechnology, University of Verona, via delle Grazie 15, 37134, Verona, Italy;Faculty of Science and Technology, Free University of Bolzano, piazza Università 5, 39100, Bolzano, Italy;

关键词: Tomato Plant;    Tomato Root;    Root Transcriptome;    Cellular Component Organization;    Haemophilus Ducreyi;   

DOI  :  10.1186/s12864-015-2331-5

 received in 2015-06-06, accepted in 2015-12-17,  发布年份 2016

来源: Springer

【 摘 要 】

BackgroundIt is well known that in the rhizosphere soluble Fe sources available for plants are mainly represented by a mixture of complexes between the micronutrient and organic ligands such as carboxylates and phytosiderophores (PS) released by roots, as well as fractions of humified organic matter. The use by roots of these three natural Fe sources (Fe-citrate, Fe-PS and Fe complexed to water-extractable humic substances, Fe-WEHS) have been already studied at physiological level but the knowledge about the transcriptomic aspects is still lacking.ResultsThe 59Fe concentration recorded after 24 h in tissues of tomato Fe-deficient plants supplied with 59Fe complexed to WEHS reached values about 2 times higher than those measured in response to the supply with Fe-citrate and Fe-PS. However, after 1 h no differences among the three Fe-chelates were observed considering the 59Fe concentration and the root Fe(III) reduction activity. A large-scale transcriptional analysis of root tissue after 1 h of Fe supply showed that Fe-WEHS modulated only two transcripts leaving the transcriptome substantially identical to Fe-deficient plants. On the other hand, Fe-citrate and Fe-PS affected 728 and 408 transcripts, respectively, having 289 a similar transcriptional behaviour in response to both Fe sources.ConclusionsThe root transcriptional response to the Fe supply depends on the nature of chelating agents (WEHS, citrate and PS). The supply of Fe-citrate and Fe-PS showed not only a fast back regulation of molecular mechanisms modulated by Fe deficiency but also specific responses due to the uptake of the chelating molecule. Plants fed with Fe-WEHS did not show relevant changes in the root transcriptome with respect to the Fe-deficient plants, indicating that roots did not sense the restored cellular Fe accumulation.

【 授权许可】

CC BY   
© Zamboni et al. 2016