Genome-wide microarray analysis of tomato roots showed defined responses to iron deficiency

BMC Genomics

Research Article

Laura Zanin¹  Nicola Tomasi¹  Roberto Pinton¹  Zeno Varanini²  Anita Zamboni²  Mario Pezzotti²  Stefano Cesco³ 

[1] Department of Agriculture and Environmental Sciences, University of Udine, via delle Scienze 208, 33100, Udine, Italy;Department of Biotechnology, University of Verona, via delle Grazie 15, 37134, Verona, Italy;Faculty of Science and Technology, Free University of Bolzano, piazza Università 5, 39100, Bolzano, Italy;

关键词: Root Hair;    Tomato Root;    Methionine Cycle;    Hair Root Production;    Root Gene Expression;   

DOI  :  10.1186/1471-2164-13-101

 received in 2011-08-03, accepted in 2012-03-20,  发布年份 2012

来源: Springer

【 摘 要 】

BackgroundPlants react to iron deficiency stress adopting different kind of adaptive responses. Tomato, a Strategy I plant, improves iron uptake through acidification of rhizosphere, reduction of Fe3+ to Fe2+ and transport of Fe2+ into the cells. Large-scale transcriptional analyses of roots under iron deficiency are only available for a very limited number of plant species with particular emphasis for Arabidopsis thaliana. Regarding tomato, an interesting model species for Strategy I plants and an economically important crop, physiological responses to Fe-deficiency have been thoroughly described and molecular analyses have provided evidence for genes involved in iron uptake mechanisms and their regulation. However, no detailed transcriptome analysis has been described so far.ResultsA genome-wide transcriptional analysis, performed with a chip that allows to monitor the expression of more than 25,000 tomato transcripts, identified 97 differentially expressed transcripts by comparing roots of Fe-deficient and Fe-sufficient tomato plants. These transcripts are related to the physiological responses of tomato roots to the nutrient stress resulting in an improved iron uptake, including regulatory aspects, translocation, root morphological modification and adaptation in primary metabolic pathways, such as glycolysis and TCA cycle. Other genes play a role in flavonoid biosynthesis and hormonal metabolism.ConclusionsThe transcriptional characterization confirmed the presence of the previously described mechanisms to adapt to iron starvation in tomato, but also allowed to identify other genes potentially playing a role in this process, thus opening new research perspectives to improve the knowledge on the tomato root response to the nutrient deficiency.

【 授权许可】

Unknown   
© Zamboni et al; licensee BioMed Central Ltd. 2012. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.