Paeoniflorin inhibits excitatory amino acid agonist-and high-dose morphine-induced nociceptive behavior in mice via modulation of N-methyl-D-aspartate receptors

BMC Complementary and Alternative Medicine

Research Article

Yu-Chien Chen¹  Ming-Ming Lee²  Hsun-Lang Fang³  Yuh-Fung Chen⁴  Huei-Yann Tsai⁵  Jhao-Guei Yang⁶ 

[1] Department of Biomedical Informatics, Asia University, No 500 Lioufeng Road, Wufeng District, 41354, Taichung, Taiwan;Department of Health and Nutrition Biotechnology, Asia University, No 500 Lioufeng Road, Wufeng District, 41354, Taichung, Taiwan;Department of Pharmacology, China Medical University, No 91, Hsueh-Shih Road, 40402, Taichung, Taiwan;Department of Pharmacology, China Medical University, No 91, Hsueh-Shih Road, 40402, Taichung, Taiwan;Department of Pharmacy, China Medical University Hospital, No 2, Yu-Der Road, 40431, Taichung, Taiwan;Department of Pharmacy, China Medical University Hospital, No 2, Yu-Der Road, 40431, Taichung, Taiwan;Laboratory of Computational and System Biology, China Medical University, Taichung, No 91, Hsueh-Shih Road, 40402, Taichung, Taiwan;

关键词: Paeoniflorin;    Excitatory amino acid agonists;    High-dose morphine;    Nociceptive behavior;    Antisense oligodeoxynucleotides;    NMDA receptor;    NR2B;   

DOI  :  10.1186/s12906-016-1230-x

 received in 2015-12-19, accepted in 2016-07-19,  发布年份 2016

来源: Springer

【 摘 要 】

BackgroundPain, the most common reasons for physician consultation, is a major symptom in many medical conditions that can significantly interfere with a person’s life quality and general functioning. Almost all painkillers have its untoward effects. Therefore, seeking for a safe medication for pain relieve is notable nowadays. Paeonia lactiflora is a well-known traditional Chinese medicine. Paeoniflorin is an active component found in Paeonia lactiflora, which has been reported to inhibit formalin-induced nociceptive behavior in mice. Aims of this present study were to investigate effects of paeoniflorin on excitatory amino acid agonist- or high-dose morphine-induced nociceptive behaviors in mice.ResultsPaeoniflorin (100, 200, 500 nmol, i.c.v.) alone and combined with glutamatergic antagonists (MK-801 14.8 pmol, or NBQX 5 nmol, i.t.) inhibited nociception. Those agents also inhibited the clonic seizure-like excitation induced by high-dose morphine (250 nmol, i.t) in mice. Antisense oligodeoxynucleotides of NMDA receptor subunits NR1, NR2A, NR2B significantly enhanced the inhibition of paeoniflorin on excitatory amino acid-and high-dose morphine-induced nociception. Docking energy data revealed that paeoniflorin had stronger binding activity in NR2A and NR2B than NR2C of NMDA receptors.ConclusionsResults of this study indicate that paeoniflorin-induced inhibition of excitatory amino acid agonist- and high-dose morphine-induced nociceptive behaviors might be due to modulation of NMDA receptors, specifically the NR2B subunit.

【 授权许可】

CC BY   
© The Author(s). 2016