A novel mutant allele of SSI2 confers a better balance between disease resistance and plant growth inhibition on Arabidopsis thaliana

BMC Plant Biology

Research Article

Jing Li¹  Zhubing Hu²  Li Liu³  Xinhua Ding⁴  Zhaohui Chu⁴  Wei Yang⁴  Yong Wang⁴  Ran Dong⁴ 

[1] College of Life Sciences, Northwest A&F University, 712100, Yangling, Shanxi, China;College of Life Sciences, Northwest A&F University, 712100, Yangling, Shanxi, China;Present address: Department of Plant Systems Biology, VIB, Ghent University, Technologiepark 927, 9052, Ghent, Belgium;Key Laboratory for Economic Plants and Biotechnology, Kunming Institute of Botany, Chinese Academy of Sciences, 650201, Kunming, China;State Key Laboratory of Crop Biology, College of Agronomy, Shandong Provincial Key Laboratory of Agricultural Microbiology, College of Plant Protection, Shandong Agricultural University, 271018, Tai’an, China;

关键词: Auxin;    Leaf shape;    Plant immunity;    Stearoyl-ACP desaturase;    Salicylic acid;   

DOI  :  10.1186/s12870-016-0898-x

 received in 2016-06-16, accepted in 2016-09-16,  发布年份 2016

来源: Springer

【 摘 要 】

BackgroundResistance and growth are opposing characteristics in plants. SA INSENSITIVITY OF npr1-5 (SSI2) encodes a stearoyl-ACP desaturase (S-ACP DES) that has previously been reported to simultaneously enhance resistance and repress growth.ResultsHere, we characterize ssi2-2, a novel mutant allele of SSI2 that has two amino acid substitutions. Compared with wild-type and two other mutants of SSI2, ssi2-2 showed intermediate phenotypes in growth size, punctate necrosis, resistance to the bacterial pathogen Pst DC3000, salicylic acid (SA) content, pathogenesis-related (PR) gene levels and 18:1 content. These results indicate that ssi2-2 is a weak mutant of SSI2. Additionally, by using ssi2-2 as an intermediate control, a number of differentially expressed genes were identified in transcriptome profiling analysis. These results suggest that constitutive expression of defense-related genes and repression of IAA signaling-associated genes is present in all SSI2 mutants.ConclusionsTaken together, our results suggest that the weak ssi2-2 mutant maintains a better balance between plant immunity and vegetative growth than other mutants, consequently providing a basis to genetically engineer disease resistance in crop plants.

【 授权许可】

CC BY   
© The Author(s). 2016