Elevation of tumour markers TGF-β, M2-PK, OV-6 and AFP in hepatocellular carcinoma (HCC)-induced rats and their suppression by microalgae Chlorella vulgaris

BMC Cancer

Research Article

Hanafi Ahmad Damanhuri¹  Suhaniza Sulaiman¹  Khaizurin Tajul Arifin¹  Yasmin Anum Mohd Yusof¹  Wan Zurinah Wan Ngah¹  Suhana Md Saad² 

[1] Department of Biochemistry, Faculty of Medicine, Universiti Kebangsaan Malaysia Medical Centre, Jalan Yaacob Latiff, 56000 Cheras, Kuala Lumpur, Wilayah Persekutuan, Malaysia;Department of Diagnostic & Allied Health Sciences, Faculty of Health & Life Sciences, Management & Science University (MSU), University Drive, Seksyen 13, 40100, Shah Alam, Selangor, Malaysia;

关键词: Chlorella vulgaris;    Liver cancer;    AFP;    TGF-β;    M-PK;    OV-6;   

DOI  :  10.1186/s12885-017-3883-3

 received in 2016-12-01, accepted in 2017-12-07,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundChlorella vulgaris (ChV), a unicellular green algae has been reported to have anticancer and antioxidant effects. The aim of this study was to determine the chemopreventive effect of ChV on liver cancer induced rats by determining the level and expression of several liver tumour markers.MethodsMale Wistar rats (200–250 g) were divided into 4 groups according to the diet given: control group (normal diet), ChV group with three different doses (50, 150 and 300 mg/kg body weight), liver cancer- induced group (choline deficient diet + 0.1% ethionine in drinking water or CDE group), and the treatment group (CDE group treated with three different doses of ChV). Rats were killed at 0, 4, 8 and 12 weeks of experiment and blood and tissue samples were taken from all groups for the determination of tumour markers expression alpha-fetoprotein (AFP), transforming growth factor-β (TGF-β), M2-pyruvate kinase (M2-PK) and specific antigen for oval cells (OV-6).ResultsSerum level of TGF-β increased significantly (p < 0.05) in CDE rats. However, ChV at all doses managed to decrease (p < 0.05) its levels to control values. Expressions of liver tumour markers AFP, TGF-β, M2-PK and OV-6 were significantly higher (p < 0.05) in tissues of CDE rats when compared to control showing an increased number of cancer cells during hepatocarcinogenesis. ChV at all doses reduced their expressions significantly (p < 0.05).ConclusionsChlorella vulgaris has chemopreventive effect by downregulating the expression of tumour markers M2-PK, OV-6, AFP and TGF-β, in HCC-induced rats.

【 授权许可】

CC BY   
© The Author(s). 2017