Clonal dissemination of linezolid-resistant Staphylococcus capitis with G2603T mutation in domain V of the 23S rRNA and the cfr gene at a tertiary care hospital in China

BMC Infectious Diseases

Research Article

Kui Zhang¹  Wanqing Zhou¹  Mingzhe Ning¹  Han Shen¹  Zhifeng Zhang¹  Xiaoli Cao¹  Dongmei Niu² 

[1] Department of Laboratory Medicine, Nanjing Drum Tower Hospital, the affiliated Hospital of Nanjing University Medical School, 321# Zhongshan Road, Gulou District, 210008, Nanjing, Jiangsu Province, PR China;Department of Laboratory Medicine, Nanjing Jinling Hospital, the affiliated Hospital of Nanjing University Medical School, 305# East Zhongshan Road, Baixia District, 210002, Nanjing, Jiangsu Province, PR China;

关键词: Linezolid;    Resistance;    cfr;    23S rRNA gene;    Staphylococcus capitis;   

DOI  :  10.1186/s12879-015-0841-z

 received in 2014-06-30, accepted in 2015-02-18,  发布年份 2015

来源: Springer

【 摘 要 】

BackgroundThe present study aims to investigate the potential mechanism of linezolid-resistant Staphylococcus capitis (LRSC) isolates collected from our hospital.MethodsThe susceptibilities of 5 Staphylococcus capitis isolates displaying resistance towards linezolid were determined by E-test. Polymerase chain reactions (PCRs) and DNA sequencing were used to investigate the potential molecular mechanism. Clonal relatedness between these strains was analyzed by pulsed-field gel electrophoresis (PFGE).ResultsThe MICs of linezolid on these 5 isolates were >256 μg/mL. The G2603T mutation was observed in the domain V of the 23S rRNA with cfr gene being also widely detected among these 5 strains. PFGE analysis displayed close genetic relatedness between these linezolid-resistant isolates.ConclusionsThe emergence of LRSC isolates carrying G2603T mutation in the domain V of the 23S rRNA and harboring cfr gene in our hospital may pose a potential challenge to the public health.

【 授权许可】

Unknown   
© Zhou et al.; licensee BioMed Central. 2015. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.