Clonal dissemination of linezolid-resistant Staphylococcus capitis with G2603T mutation in domain V of the 23S rRNA and the cfr gene at a tertiary care hospital in China

BMC Infectious Diseases

Kui Zhang¹  Han Shen¹  Zhifeng Zhang¹  Mingzhe Ning¹  Xiaoli Cao¹  Dongmei Niu²  Wanqing Zhou¹ 

[1] Department of Laboratory Medicine, Nanjing Drum Tower Hospital, the affiliated Hospital of Nanjing University Medical School, 321# Zhongshan Road, Gulou District, Nanjing 210008, Jiangsu Province, PR China;Department of Laboratory Medicine, Nanjing Jinling Hospital, the affiliated Hospital of Nanjing University Medical School, 305# East Zhongshan Road, Baixia District, Nanjing 210002, Jiangsu Province, PR China

关键词: Staphylococcus capitis;    23S rRNA gene;    cfr gene;    Resistance;    Linezolid;   

Others  :  1135693
DOI  :  10.1186/s12879-015-0841-z

 received in 2014-06-30, accepted in 2015-02-18,  发布年份 2015

【 摘 要 】

Background

The present study aims to investigate the potential mechanism of linezolid-resistant Staphylococcus capitis (LRSC) isolates collected from our hospital.

Methods

The susceptibilities of 5 Staphylococcus capitis isolates displaying resistance towards linezolid were determined by E-test. Polymerase chain reactions (PCRs) and DNA sequencing were used to investigate the potential molecular mechanism. Clonal relatedness between these strains was analyzed by pulsed-field gel electrophoresis (PFGE).

Results

The MICs of linezolid on these 5 isolates were >256 μg/mL. The G2603T mutation was observed in the domain V of the 23S rRNA with cfr gene being also widely detected among these 5 strains. PFGE analysis displayed close genetic relatedness between these linezolid-resistant isolates.

Conclusions

The emergence of LRSC isolates carrying G2603T mutation in the domain V of the 23S rRNA and harboring cfr gene in our hospital may pose a potential challenge to the public health.

【 授权许可】

   
2015 Zhou et al.; licensee BioMed Central.

【 图 表 】

Figure 1.

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