RNA sequencing-based analysis of the spleen transcriptome following infectious bronchitis virus infection of chickens selected for different mannose-binding lectin serum concentrations

BMC Genomics

Research Article

Jun Chen¹  Rikke Brødsgaard Kjærup²  Tina Sørensen Dalgaard²  Helle Risdahl Juul-Madsen²  Bart Buitenhuis³  Eva Wattrang⁴  Valentina Gualdi⁵  Francesco Strozzi⁵  John L. Williams⁶  Guilietta Minozzi⁷  Edin Hamzić⁸  Núria Mach⁹ 

[1] Cobb-Vantress Inc, US-412 Road, 72761, Siloam Springs, AR, USA;Department of Animal Science, Aarhus University, P.O. Box 50, Blichers Allé 20, 8830, Tjele, Denmark;Department of Molecular Biology and Genetics, Center for Quantitative Genetics and Genomics, Aarhus University, P.O. Box 50, Blichers Allé 20, 8830, Tjele, Denmark;National Veterinary Institute, Ulls väg 2B, 751 89, Uppsala, Sweden;Parco Tecnologico Padano, Via Einstein, 26900, Lodi, Italy;Parco Tecnologico Padano, Via Einstein, 26900, Lodi, Italy;School of Animal and Veterinary Sciences, University of Adelaide, 5371, Roseworthy, SA, Australia;Parco Tecnologico Padano, Via Einstein, 26900, Lodi, Italy;University of Milan, DIVET, Via Celoria 10, 20133, Milan, Italy;UMR1313 Animal Genetics and Integrative Biology Unit, AgroParisTech, Université Paris-Saclay, 16 rue Claude Bernard, 75005, Paris, France;UMR1313 Animal Genetics and Integrative Biology Unit, INRA, Université Paris-Saclay, Domaine de Vilvert, 78350, Jouy-en-Josas, France;Department of Molecular Biology and Genetics, Center for Quantitative Genetics and Genomics, Aarhus University, P.O. Box 50, Blichers Allé 20, 8830, Tjele, Denmark;UMR1313 Animal Genetics and Integrative Biology Unit, INRA, Université Paris-Saclay, Domaine de Vilvert, 78350, Jouy-en-Josas, France;

关键词: IBV;    Coronavirus;    Infectious bronchitis;    Chicken;    RNA sequencing;    Transcriptome;    Spleen;    Mannose-binding lectin;    Immune response;   

DOI  :  10.1186/s12864-016-2403-1

 received in 2015-08-24, accepted in 2016-01-18,  发布年份 2016

来源: Springer

【 摘 要 】

BackgroundAvian infectious bronchitis is a highly contagious disease of the upper-respiratory tract caused by infectious bronchitis virus (IBV). Understanding the molecular mechanisms involved in the interaction between innate and adaptive immune responses to IBV infection is a crucial element for further improvements in strategies to control IB. To this end, two chicken lines, selected for high (L10H line) and low (L10L line) serum concentration of mannose-binding lectin (MBL) were studied. In total, 32 birds from each line were used. Sixteen birds from each line were infected with IBV and sixteen were left uninfected. Eight uninfected and infected birds from each line were euthanized at 1 and 3 weeks post infection. RNA sequencing was performed on spleen samples from all 64 birds and differential gene expression analysis was performed for four comparisons: L10L line versus L10H line for uninfected birds at weeks 1 and 3, respectively, and in the same way for infected birds. Functional analysis was performed using Gene Ontology (GO) Immune System Process terms specific for Gallus gallus.ResultsComparing uninfected L10H and L10L birds, we identified 1698 and 1424 differentially expressed (DE) genes at weeks 1 and 3, respectively. For the IBV-infected birds, 1934 and 866 DE genes were identified between the two lines at weeks 1 and 3, respectively. The two most enriched GO terms emerging from the comparison of uninfected birds between the two lines were “Lymphocyte activation involved in immune response” and “Somatic recombination of immunoglobulin genes involved in immune response” at weeks 1 and 3, respectively. When comparing IBV-infected birds between the two lines, the most enriched GO terms were “Alpha-beta T cell activation” and “Positive regulation of leukocyte activation” at weeks 1 and 3, respectively.ConclusionsHealthy birds from the two lines showed significant differences in expression profiles for subsets of adaptive and innate immunity-related genes, whereas comparison of the IBV-infected birds from the two lines showed differences in expression of immunity-related genes involved in T cell activation and proliferation. The observed transcriptome differences between the two lines indicate that selection for MBL had influenced innate as well as adaptive immunity.

【 授权许可】

CC BY   
© Hamzić et al. 2016