Potential dermal wound healing agent in Blechnum orientale Linn

BMC Complementary and Alternative Medicine

Research Article

Kah Hwi Kim¹  How Yee Lai²  Yau Yan Lim³ 

[1] Department of Physiology, Faculty of Medicine, University of Malaya, 50603, Kuala Lumpur, Malaysia;School of Biosciences, Taylor's University Lakeside Campus, No. 1, Jalan Taylor's, 47500, Subang Jaya, Selangor, Malaysia;School of Science, Monash University Sunway Campus, Bandar Sunway, 46150, Petaling Jaya, Selangor, Malaysia;

关键词: Water Extract;    Vehicle Group;    Hydroxyproline Content;    Wound Contraction;    Manuka Honey;   

DOI  :  10.1186/1472-6882-11-62

 received in 2011-05-23, accepted in 2011-08-12,  发布年份 2011

来源: Springer

【 摘 要 】

BackgroundBlechnum orientale Linn. (Blechnaceae) is used ethnomedicinally to treat wounds, boils, blisters or abscesses and sores, stomach pain and urinary bladder complaints. The aim of the study was to validate the ethnotherapeutic claim and to evaluate the effects of B. orientale water extract on wound healing activity.MethodsWater extract of B. orientale was used. Excision wound healing activity was examined on Sprague-Dawley rats, dressed with 1% and 2% of the water extract. Control groups were dressed with the base cream (vehicle group, negative control) and 10% povidone-iodine (positive control) respectively. Healing was assessed based on contraction of wound size, mean epithelisation time, hydroxyproline content and histopathological examinations. Statistical analyses were performed using one way ANOVA followed by Tukey HSD test.ResultsWound healing study revealed significant reduction in wound size and mean epithelisation time, and higher collagen synthesis in the 2% extract-treated group compared to the vehicle group. These findings were supported by histolopathological examinations of healed wound sections which showed greater tissue regeneration, more fibroblasts and angiogenesis in the 2% extract-treated group.ConclusionsThe ethnotherapeutic use of this fern is validated. The water extract of B. orientale is a potential candidate for the treatment of dermal wounds. Synergistic effects of both strong antioxidant and antibacterial activities in the extract are deduced to have accelerated the wound repair at the proliferative phase of the healing process.

【 授权许可】

Unknown   
© Lai et al; licensee BioMed Central Ltd. 2011. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.