Tannin extracts from immature fruits of Terminalia chebula Fructus Retz. promote cutaneous wound healing in rats

BMC Complementary and Alternative Medicine

Research Article

Kun Li¹  Bo Yu¹  Hong Yang¹  Shouyu Wang²  Yunpeng Diao²  Zhen Zhang²  Shanshan Huang²  Houli Zhang² 

[1] College of Life Sciences, Liaoning Normal University, 116029, Dalian, P.R. China;College of Pharmacy, Dalian Medical University, 116044, Dalian, P.R. China;

关键词: Minimum Bactericidal Concentration;    VEGFA Expression;    Immature Fruit;    Wound Contraction;    Klebsiella Pneumonia;   

DOI  :  10.1186/1472-6882-11-86

 received in 2011-06-01, accepted in 2011-10-07,  发布年份 2011

来源: Springer

【 摘 要 】

BackgroundTannins extracted from immature fruits of Terminalia chebula Fructus Retz. are considered as effective components promoting the process of wound healing. The objective of this study is to explore the optimal extraction and purification technology (OEPT) of tannins, while studying the use of this drug in the treatment of a cutaneous wound of rat as well as its antibacterial effects.MethodsThe content of tannin extracts was measured by the casein method, and antibacterial ability was studied by the micro-dilution method in vitro. In wound healing experiment, animals in group I, II and II were treated with vaseline ointment, tannin extracts (tannin content: 81%) and erythromycin ointment, respectively (5 mg of ointment were applied on each wound). To evaluate the process of wound healing, selected pharmacological and biochemical parameters were applied.ResultsAfter optimal extraction and purification, content of tannin extracts was increased to 81%. Tannin extracts showed the inhibition of Staphylococcus aureus and Klebsiella Pneumonia in vitro. After excision of wounds, on days 7 and 10, the percent of wound contraction of groupwas higher than that of group . After being hurt with wounds, on days 3, 7, and 10, the wound healing quality of groupwas found to be better than that of groupin terms of granulation formation and collagen organization. After wound creation, on day 3, the vascular endothelial growth factor expression of groupwas higher than that of group .ConclusionThe results suggest that tannin extracts from dried immature fruits of Terminalia chebula Fructus Retz. can promote cutaneous wound healing in rats, probably resulting from a powerful anti-bacterial and angiogenic activity of the extracts.

【 授权许可】

Unknown   
© Li et al; licensee BioMed Central Ltd. 2011. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.