BMC Infectious Diseases | |
A novel multi-epitope recombined protein for diagnosis of human brucellosis | |
Research Article | |
Yushen Liu1  Dehui Yin1  Juan Wang1  Juan Li1  Dandan Song1  Weiyi Song1  Li Li1  Wen Ju1  Xiangjun Meng1  Hongqian Cao1  Kun Xu1  Xiaofeng Qu1  Xiuling Song1  Han Li2  Rizeng Meng3  Jinhua Liu4  | |
[1] Department of Health Laboratory, School of Public Health, Jilin University, Changchun, China;Department of Health Laboratory, School of Public Health, Jilin University, Changchun, China;Department of Infection Control, First Hospital of Jilin University, Changchun, China;Department of Health Laboratory, School of Public Health, Jilin University, Changchun, China;Jilin Entry-Exit Inspection and Quarantine Bureau, Changchun, China;Jilin Entry-Exit Inspection and Quarantine Bureau, Changchun, China; | |
关键词: Brucellosis; Diagnosis; Recombinant protein; | |
DOI : 10.1186/s12879-016-1552-9 | |
received in 2015-08-20, accepted in 2016-05-07, 发布年份 2016 | |
来源: Springer | |
【 摘 要 】
BackgroundIn epidemic regions of the world, brucellosis is a reemerging zoonosis with minimal mortality but is a serious public hygiene problem. Currently, there are various methods for brucellosis diagnosis, however few of them are available to be used to diagnose, especially for serious cross-reaction with other bacteria.MethodTo overcome this disadvantage, we explored a novel multi-epitope recombinant protein as human brucellosis diagnostic antigen. We established an indirect enzyme-linked immunosorbent assay (ELISA) based on this recombinant protein. 248 sera obtained from three different groups including patients with brucellosis (146 samples), non-brucellosis patients (82 samples), and healthy individuals (20 samples) were tested by indirect ELISA. To evaluate the assay, a receiver-operating characteristic (ROC) analysis and immunoblotting were carried out using these characterized serum samples.ResultsFor this test, the area under the ROC curve was 0.9409 (95 % confidence interval, 0.9108 to 0.9709), and a sensitivity of 88.89 % and a specificity of 85.54 % was given with a cutoff value of 0.3865 from this ROC analysis. The Western blot results indicate that it is feasible to differentiate human brucellosis and non-brucellosis with the newly established method based on this recombinant protein.ConclusionOur results obtained high diagnostic accuracy of the ELISA assay which encourage the use of this novel recombinant protein as diagnostic antigen to implement serological diagnosis of brucellosis.
【 授权许可】
CC BY
© Yin et al. 2016
【 预 览 】
Files | Size | Format | View |
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RO202311096216689ZK.pdf | 942KB | download |
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