Mapping the sex determination locus in the Atlantic halibut (Hippoglossus hippoglossus) using RAD sequencing

BMC Genomics

Research Article

Münevver Oral¹  Christos Palaiokostas¹  Mairi E Cowan¹  Andrew Davie¹  Hervé Migaud¹  Brendan J McAndrew¹  John B Taggart¹  David J Penman¹  Michaël Bekaert¹  Karim Gharbi² 

[1] Institute of Aquaculture, School of Natural Sciences, University of Stirling, FK9 4LA, Stirling, Scotland, UK;The GenePool, School of Biological Sciences, University of Edinburgh, EH9 3JT, Edinburgh, Scotland, UK;

关键词: Hippoglossus hippoglossus;    Sex determination;    Monosex;    QTL mapping;    RAD-seq;    Aquaculture;   

DOI  :  10.1186/1471-2164-14-566

 received in 2013-02-01, accepted in 2013-08-16,  发布年份 2013

来源: Springer

【 摘 要 】

BackgroundAtlantic halibut (Hippoglossus hippoglossus) is a high-value, niche market species for cold-water marine aquaculture. Production of monosex female stocks is desirable in commercial production since females grow faster and mature later than males. Understanding the sex determination mechanism and developing sex-associated markers will shorten the time for the development of monosex female production, thus decreasing the costs of farming.ResultsHalibut juveniles were masculinised with 17 α-methyldihydrotestosterone (MDHT) and grown to maturity. Progeny groups from four treated males were reared and sexed. Two of these groups (n = 26 and 70) consisted of only females, while the other two (n = 30 and 71) contained balanced sex ratios (50% and 48% females respectively). DNA from parents and offspring from the two mixed-sex families were used as a template for Restriction-site Associated DNA (RAD) sequencing. The 648 million raw reads produced 90,105 unique RAD-tags. A linkage map was constructed based on 5703 Single Nucleotide Polymorphism (SNP) markers and 7 microsatellites consisting of 24 linkage groups, which corresponds to the number of chromosome pairs in this species. A major sex determining locus was mapped to linkage group 13 in both families. Assays for 10 SNPs with significant association with phenotypic sex were tested in both population data and in 3 additional families. Using a variety of machine-learning algorithms 97% correct classification could be obtained with the 3% of errors being phenotypic males predicted to be females.ConclusionAltogether our findings support the hypothesis that the Atlantic halibut has an XX/XY sex determination system. Assays are described for sex-associated DNA markers developed from the RAD sequencing analysis to fast track progeny testing and implement monosex female halibut production for an immediate improvement in productivity. These should also help to speed up the inclusion of neomales derived from many families to maintain a larger effective population size and ensure long-term improvement through selective breeding.

【 授权许可】

Unknown   
© Palaiokostas et al.; licensee BioMed Central Ltd. 2013. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.