A high-density linkage map and sex-linked markers for the Amazon Tambaqui Colossoma macropomum

BMC Genomics

Fernanda Loureiro de Almeida¹  Luiz Eduardo Lima de Freitas²  Eduardo Sousa Varela²  Luciana Shiotsuki²  Aurisan da Silva Barroso²  Lucas Simon Torati²  Luciana Cristine Vasques Villela²  Luciana Nakaghi Ganeco-Kirschnik²  Fabrício Pereira Rezende²  John Bernard Taggart³  Herve Migaud³  Michaël Bekaert³ 

[1] Embrapa Amazônia Ocidental, CEP 69010-970, Manaus, AM, Brazil;Embrapa Pesca e Aquicultura, Prolongamento da Av. NS 10, Cruzamento com AV. LO 18, Sentido Norte, loteamento Água Fria, CEP, 77008-900, Palmas, TO, Brazil;Institute of Aquaculture, Faculty of Natural Sciences, University of Stirling, FK9 4LA, Stirling, Scotland, UK;

关键词: Linkage map;    Sex-linked markers;    Monosex;    Sex determination;    Colossoma macropomum;    Aquaculture;   

DOI  :  10.1186/s12864-021-08037-8

来源: Springer

【 摘 要 】

BackgroundTambaqui (Colossoma macropomum, Cuvier, 1818) is the most economically important native freshwater fish species in Brazil. It can reach a total length of over 1 m and a weight of over 40 kg. The species displays a clear sex dimorphism in growth performance, with females reaching larger sizes at harvest. In aquaculture, the production of monosex populations in selective breeding programmes has been therefore identified as a key priority.ResultsIn the present study, a genetic linkage map was generated by double digest restriction-site associated DNA (ddRAD) sequencing from 248 individuals sampled from two F1 families. The map was constructed using 14,805 informative SNPs and spanned 27 linkage groups. From this, the tambaqui draft genome was improved, by ordering the scaffolds into chromosomes, and sex-linked markers were identified. A total of 235 markers on linkage group 26 showed a significant association with the phenotypic sex, supporting an XX/XY sex determination system in the species. The four most informative sex-linked markers were validated on another 206 sexed individuals, demonstrating an accuracy in predicting sex ranging from 90.0 to 96.7%.ConclusionsThe genetic mapping and novel sex-linked DNA markers identified and validated offer new tools for rapid progeny sexing, thus supporting the development of monosex female production in the industry while also supporting breeding programmes of the species.

【 授权许可】

CC BY