Identification of mildew resistance in wild and cultivated Central Asian grape germplasm

BMC Plant Biology

Research Article

Summaira Riaz¹  Alan C Tenscher¹  M Andrew Walker¹  Thierry Lacombe²  Valerie Laucou²  Jean-Michel Boursiquot³  Gerald S Dangl⁴ 

[1] Department of Viticulture and Enology, University of California, 95616, Davis, CA, USA;Equipe Diversité et Adaptation de la Vigne et des Espèces Méditerranéennes, UMR AGAP, INRA, 2 Place Viala, 34060, Montpellier, France;Equipe Diversité et Adaptation de la Vigne et des Espèces Méditerranéennes, UMR AGAP, Montpellier SupAgro, 2 Place Viala, 34060, Montpellier, France;Foundation Plant Services, University of California, 95616, Davis, CA, USA;

关键词: Powdery mildew resistance;    Vitis vinifera;    Vitis vinifera;    Gene flow;   

DOI  :  10.1186/1471-2229-13-149

 received in 2013-03-18, accepted in 2013-09-30,  发布年份 2013

来源: Springer

【 摘 要 】

BackgroundCultivated grapevines, Vitis vinifera subsp. sativa, evolved from their wild relative, V. vinifera subsp. sylvestris. They were domesticated in Central Asia in the absence of the powdery mildew fungus, Erysiphe necator, which is thought to have originated in North America. However, powdery mildew resistance has previously been discovered in two Central Asian cultivars and in Chinese Vitis species.ResultsA set of 380 unique genotypes were evaluated with data generated from 34 simple sequence repeat (SSR) markers. The set included 306 V. vinifera cultivars, 40 accessions of V. vinifera subsp. sylvestris, and 34 accessions of Vitis species from northern Pakistan, Afghanistan and China. Based on the presence of four SSR alleles previously identified as linked to the powdery mildew resistance locus, Ren1, 10 new mildew resistant genotypes were identified in the test set: eight were V. vinifera cultivars and two were V. vinifera subsp. sylvestris based on flower and seed morphology. Sequence comparison of a 620 bp region that includes the Ren1-linked allele (143 bp) of the co-segregating SSR marker SC8-0071-014, revealed that the ten newly identified genotypes have sequences that are essentially identical to the previously identified mildew resistant V. vinifera cultivars: ‘Kishmish vatkana’ and ‘Karadzhandal’. Kinship analysis determined that three of the newly identified powdery mildew resistant accessions had a relationship with ‘Kishmish vatkana’ and ‘Karadzhandal’, and that six were not related to any other accession in this study set. Clustering procedures assigned accessions into three groups: 1) Chinese species; 2) a mixed group of cultivated and wild V. vinifera; and 3) table grape cultivars, including nine of the powdery mildew resistant accessions. Gene flow was detected among the groups.ConclusionsThis study provides evidence that powdery mildew resistance is present in V. vinifera subsp. sylvestris, the dioecious wild progenitor of the cultivated grape. Four first-degree parent progeny relationships were discovered among the hermaphroditic powdery mildew resistant cultivars, supporting the existence of intentional grape breeding efforts. Although several Chinese grape species are resistant to powdery mildew, no direct genetic link to the resistance found in V. vinifera could be established.

【 授权许可】

Unknown   
© Riaz et al.; licensee BioMed Central Ltd. 2013. This article is published under license to BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.