BMC Neuroscience | |
Schwann cells migrate along axons in the absence of GDNF signaling | |
Research Article | |
Katalin Zajzon1  Björn Spittau1  Kerstin Krieglstein2  Stephan Heermann3  Markus H Schwab4  | |
[1] Department of Molecular Embryology Institute of Anatomy and Cell Biology, University of Freiburg, Freiburg, Germany;Department of Molecular Embryology Institute of Anatomy and Cell Biology, University of Freiburg, Freiburg, Germany;FRIAS, University of Freiburg, Freiburg, Germany;Department of Neuroanatomy, University of Heidelberg, Heidelberg, Germany;Department of Molecular Embryology Institute of Anatomy and Cell Biology, University of Freiburg, Freiburg, Germany;COS Heidelberg, INF, 230 69120, Heidelberg, Germany;Department of Neurogenetics, Max Planck Institute of Experimental Medicine, Göttingen, Germany; | |
关键词: Schwann cell development; Migration; Proliferation; GDNF; PP2; | |
DOI : 10.1186/1471-2202-13-92 | |
received in 2012-06-12, accepted in 2012-07-19, 发布年份 2012 | |
来源: Springer | |
【 摘 要 】
BackgroundDuring development neural crest derived Schwann Cell (SC) precursors migrate to nerve trunks and populate nascent nerves. Axonal ensheathment by SC is a prerequisite for normal nerve function and the integrity of myelinated as well as nonmyelinated axons. To provide adequate support functions, SC colonize entire nerves. One important prerequisite for this is their migration into distal axonal regions.ResultsHere, we studied the role of Glial cell line derived neurotrophic factor (GDNF), a TGF-beta related growth factor, for SC migration. To this end we used a superior cervical ganglion (SCG) explant-SC migration assay, GDNF null mutant mouse embryos and a chemical inhibitor for GDNF signaling in combination with time-lapse imaging. We found that GDNF signaling is dispensable for SC migration along murine embryonic sympathetic axons. Furthermore, in vivo analyzes revealed that SC migration along the sciatic nerve is also not dependent on GDNF.ConclusionsIn contrast to previous in vitro findings in the sciatic nerve and a SC precursor cell line, our results clearly indicate that GDNF is dispensable for embryonic SC migration. This is demonstrated for the sympathetic nervous system and also for the sciatic nerve in mouse.
【 授权许可】
CC BY
© Heermann et al.; licensee BioMed Central Ltd. 2012
【 预 览 】
Files | Size | Format | View |
---|---|---|---|
RO202311095909105ZK.pdf | 2437KB | download |
【 参考文献 】
- [1]
- [2]
- [3]
- [4]
- [5]
- [6]
- [7]
- [8]
- [9]
- [10]
- [11]
- [12]
- [13]
- [14]
- [15]
- [16]
- [17]
- [18]
- [19]
- [20]
- [21]
- [22]
- [23]
- [24]
- [25]
- [26]
- [27]
- [28]
- [29]
- [30]
- [31]
- [32]
- [33]