Schwann cells migrate along axons in the absence of GDNF signaling

BMC Neuroscience

Kerstin Krieglstein⁴  Markus H Schwab²  Katalin Zajzon¹  Björn Spittau¹  Stephan Heermann³ 

[1] Department of Molecular Embryology Institute of Anatomy and Cell Biology, University of Freiburg, Freiburg, Germany;Department of Neurogenetics, Max Planck Institute of Experimental Medicine, Göttingen, Germany;Current address: COS Heidelberg, INF, 230 69120, Heidelberg, Germany;FRIAS, University of Freiburg, Freiburg, Germany

关键词: PP2;    GDNF;    Proliferation;    Migration;    Schwann cell development;   

Others  :  1170589
DOI  :  10.1186/1471-2202-13-92

 received in 2012-06-12, accepted in 2012-07-19,  发布年份 2012

【 摘 要 】

Background

During development neural crest derived Schwann Cell (SC) precursors migrate to nerve trunks and populate nascent nerves. Axonal ensheathment by SC is a prerequisite for normal nerve function and the integrity of myelinated as well as nonmyelinated axons. To provide adequate support functions, SC colonize entire nerves. One important prerequisite for this is their migration into distal axonal regions.

Results

Here, we studied the role of Glial cell line derived neurotrophic factor (GDNF), a TGF-beta related growth factor, for SC migration. To this end we used a superior cervical ganglion (SCG) explant-SC migration assay, GDNF null mutant mouse embryos and a chemical inhibitor for GDNF signaling in combination with time-lapse imaging. We found that GDNF signaling is dispensable for SC migration along murine embryonic sympathetic axons. Furthermore, in vivo analyzes revealed that SC migration along the sciatic nerve is also not dependent on GDNF.

Conclusions

In contrast to previous in vitro findings in the sciatic nerve and a SC precursor cell line, our results clearly indicate that GDNF is dispensable for embryonic SC migration. This is demonstrated for the sympathetic nervous system and also for the sciatic nerve in mouse.

【 授权许可】

   
2012 Heermann et al.; licensee BioMed Central Ltd.

【 图 表 】

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