Suppression of neuropeptide production by quercetin in allergic rhinitis model rats

BMC Complementary and Alternative Medicine

Research Article

Tomomi Mizuyoshi¹  Hitome Kobayashi¹  Misako Kashiwabara²  Kazuhito Asano³ 

[1] Department of Otolaryngology, School of Medicine, Showa University, Shinagawa-ku, 142-8555, Tokyo, Japan;Graduate School of Nursing and Rehabilitation Sciences, Showa University Graduate School, Midori-ku, 226-8555, Yokohama, Japan;Graduate School of Nursing and Rehabilitation Sciences, Showa University Graduate School, Midori-ku, 226-8555, Yokohama, Japan;Division of Physiology, School of Nursing and Rehabilitation Sciences, Showa University, 1865 Touka-Ichiba, Midori-Ku, 226-8555, Yokohama, Japan;

关键词: Quercetin;    Substance P;    Calcitonin gene-related peptide;    Nerve growth factor;    Nasal allergy-like symptom;    Suppression;    Rat;   

DOI  :  10.1186/s12906-016-1123-z

 received in 2015-11-26, accepted in 2016-05-13,  发布年份 2016

来源: Springer

【 摘 要 】

BackgroundQuercetin, a dietary flavonoid found in many fruits, red wine and onion, among others, has been reported to have potent anti-oxidant, anti-viral and anti-cancer effects. Although quercetin is also reported to have anti-inflammatory and anti-allergic effects, the precise mechanisms by which quercetin favorably modify the clinical conditions of allergic diseases such as allergic rhinitis (AR). The present study was designed to examine the influence of quercetin on the development of AR by using AR model rats.MethodsSprague-Dawley (SD) rats were sensitized with toluene 2,4-diisocyanate (TDI) by intranasal instillation of a 10 % TDI in ethyl acetate in a volume of 5 μl once a day for 5 consecutive days. This sensitization procedure was repeated after a 2-day interval. After 5 days of the second sensitization, rats were treated with various doses of quercetin once a day for 2 to 7 days. Nasal allergy-like symptoms, which were induced by bilateral application of 5 μl of 10 % TDI in ethyl acetate, were assessed by counting sneezing and nasal rubbing behaviors for 10 min just after TDI nasal challenge. The levels of substance P (SP), calcitonin gene-related peptide (CGRP) and nerve growth factor (NGF) in nasal lavage fluids obtained 6 h after TDI nasal challenge was examined by ELISA.ResultsOral administration of quercetin for 5 and 7 days, but not 2 and 3 days, could inhibit sneezing and nasal rubbing movements, which were increased by TDI nasal challenge. The minimum dose that caused significant inhibition was 25 mg/kg. Oral administration of quercetin at more than 25 mg/kg for 5 days significantly inhibited the increase in SP, CGRP and NGF contents in nasal lavage fluids induced by TDI nasal challenge.ConclusionThe present results strongly suggested that quercetin will be a good candidate for the supplement on the management and treatment of allergic diseases, especially AR.

【 授权许可】

CC BY   
© Kashiwabara et al. 2016