In Vivo anti-trypanosomal activity of dichloromethane and methanol crude leaf extracts of Dovyalis abyssinica (Salicaceae) against Trypanosoma congolense

BMC Complementary and Alternative Medicine

Research Article

Nigatu Kebede¹  Getachew Terefe²  Workineh Shibeshi³  Belay Tadesse⁴ 

[1] Aklilu Lemma Institute of Pathobiology, Addis Ababa University, Addis Ababa, Ethiopia;Department of Pathology and Parasitology, College of Veterinary Medicine and Agriculture, Addis Ababa University, P.O.Box 34, Debrezeit, Ethiopia;Department of Pharmacology and Clinical Pharmacy, School of Pharmacy, College of Health Sciences, Addis Ababa University, P.O. Box 9086, Addis Ababa, Ethiopia;School of Medicine, College of Medicine and Health Sciences, Debre Markos University, P.O.Box 269, Debremarkos, Ethiopia;

关键词: Dovyalis abyssinica;    Anitrypanosomal activity;    Mice;    Trypanosoma congolense;   

DOI  :  10.1186/s12906-015-0809-y

 received in 2015-05-14, accepted in 2015-08-07,  发布年份 2015

来源: Springer

【 摘 要 】

BackgroundAfrican trypanosomiasis affects both humans and livestock in sub-Saharan countries including Ethiopia. Due to limitations to current chemotherapy, there is an urgent need for the development of new, safe, cheap and effective drugs. In the present study, the leaf of Dovyalis abyssinica was tested for its in vivo antitrypanosomal activity against Trypanosoma congolense field isolate on mice.MethodsThe leaf of D. abyssinica was macerated using dichloromethane and methanol. The extracts at doses of 250, 200, 150 and 100 mg/kg body weight were administered intraperitonealy daily for 7 days to mice infected with T. congolense. Following administration, parasitemia, packed cell volume, rectal temperature, body weight and survival time were monitored.ResultsAdministration of dichloromethane and methanol extracts at 250 and 200 mg/kg reduced (p < 0.05) parasitemia and rectal temperature, and improved (p < 0.05) PCV, mean body weight, and mean survival time compared to dimethylsulfoxide treatment.ConclusionCrude dichloromethane and methanol leaf extracts of D. abyssinica displayed anti-trypanosomal activity that may serve as lead for the development of effective alternative antitrypanosomal drugs.

【 授权许可】

CC BY   
© Tadesse et al. 2015