Time course of collagen peak in bile duct-ligated rats

BMC Gastroenterology

Research Article

Orhan Tarcin¹  Nurdan Tözün²  Metin Basaranoglu³  Gilbert Hilman⁴  Gülgün Tahan⁴  Gagan Sood⁵  Ned Snyder⁵  Cigdem Celikel⁶  Nevin Yilmaz⁷  Ilker Sücüllü⁸  Veysel Tahan⁹ 

[1] Acibadem Hospital, 34500, Istanbul, Turkey;Division of Gastroenterology Acıbadem University, 34500, Istanbul, Turkey;Division of Gastroenterology and Hepatology, Consulting, Ankara Yüksek Ihtisas Hospital Gastroenterology Clinic, 06420, Sihhiye, Ankara, Turkey;Division of Gastroenterology, Marmara University Istanbul, 34500, Turkey;Division of Gastroenterology, University of Texas Medical Branch, Galveston, USA;Division of Pathology Marmara University, 34500, Istanbul, Turkey;Division of Pharmacology, University of Texas Medical Branch, Galveston, USA;Gülhane Military Medical Faculty, Haydarpasa Educational Hospital Istanbul, 34500, Turkey;Institute of Gastroenterology, Surgery Unit, Marmara University Istanbul, 34500, Turkey;

关键词: Bile duct-ligated;    rat;    fibrosis;    fibrogenesis;    liver;    collagen;    reversible;   

DOI  :  10.1186/1471-230X-11-45

 received in 2010-10-16, accepted in 2011-04-28,  发布年份 2011

来源: Springer

【 摘 要 】

BackgroundOne of the most useful experimental fibrogenesis models is the "bile duct-ligated rats". Our aim was to investigate the quantitative hepatic collagen content by two different methods during the different stages of hepatic fibrosis in bile duct-ligated rats on a weekly basis. We questioned whether the 1-wk or 4-wk bile duct-ligated model is suitable in animal fibrogenesis trials.MethodsOf the 53 male Wistar rats, 8 (Group 0) were used as a healthy control group. Bile duct ligation (BDL) had been performed in the rest. Bile duct-ligated rates were sacrificed 7 days later in group 1 (10 rats), 14 days later in group 2 (9 rats), 21 days later in group 3(9 rats) and 28 days later in group 4 (9 rats). Eight rats underwent sham-operation (Sham). Hepatic collagen measurements as well as serum levels of liver enzymes and function tests were all analysed.ResultsThe peak level of collagen was observed biochemically and histomorphometricly at the end of third week (P < 0.001 and P < 0.05). Suprisingly, collagen levels had decreased with the course of time such as at the end of fourth week (P < 0.01 and P < 0.05).ConclusionWe have shown that fibrosis in bile duct-ligated rats is transient, i.e. reverses spontaneously after 3 weeks. This contrasts any situation in patients where hepatic fibrosis is progressive and irreversible as countless studies performed by many investigators in the same animal model.

【 授权许可】

CC BY   
© Tarcin et al; licensee BioMed Central Ltd. 2011